Human echovirus 21 (ATCC® VR-51)

Classification: Picornaviridae, Enterovirus, Human enterovirus B  /  Product Format: frozen

Classification Picornaviridae, Enterovirus, Human enterovirus B
Deposited As Echovirus 21
Agent Human echovirus 21
Strain Farina [E26D]
Biosafety Level 2
Isolation
Feces of child with aseptic meningitis, Massachusetts, 1950
Product Format frozen
Storage Conditions -60°C or colder
Comments
CPE appears later and is less marked in MkK than in HAm cell cultures but the titer and CPE increase on serial passage.
CPE appears later and is less marked in MkK than in HAm cell cultures but the titer and CPE increase on serial passage.
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
CPE
Recommended Host
human amnion cells

Host of Choice:  HAm cells

Host Range:  HAm, MkK, HEK, human embryonic skin-muscle, HED cells

human amnion; monkey kidney cells; human embryonic kidney cells; human embryonic skin and muscle; human diploid embryonic cells
Growth Conditions
Duration: 2-4 days
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
CPE
Name of Depositor S Kibrick
Source
Feces of child with aseptic meningitis, Massachusetts, 1950
Year of Origin 1950
References

Robbins FC, et al. Studies on the cultivation of poliomyelitis viruses in tissue culture. V. The direct isolation and serologic identification of virus strains in tissue culture from patients with nonparalytic and paralytic poliomyelitis. Am. J. Hyg. 54: 286-293, 1951. PubMed: 14877809