Vaccinia virus ts mutant (ATCC® VR-3189)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen

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Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus ts mutant
Strain IHD-W Dts60
Applications
The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location A24, the 132 kDa RNA polymerase subunit.
Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses.
Biosafety Level 2
Isolation
This conditional lethal mutant, originally designated plaque isolate number 7672, was isolated from the IHD-W strain of Vaccinia by S. Dales. It was not described in the original publication, but is presumed to have been selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. It has not been assigned to a Dales EM category. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location A24, the 132 kDa RNA polymerase subunit. The non-permissive incubation temperature is 39.5oC.
Product Format frozen
Storage Conditions  -70°C or colder
Comments
This conditional lethal mutant, originally designated plaque isolate number 7672, was isolated from the IHD-W strain of Vaccinia by S. Dales. It was not described in the original publication, but is presumed to have been selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. It has not been assigned to a Dales EM category. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location A24, the 132 kDa RNA polymerase subunit. The non-permissive incubation temperature is 39.5oC.
Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses.
Effect on Host
Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing.
Growth Medium ATCC® Medium 30-2002: Dulbecco's Modified Eagle's Medium (DMEM)
Fetal Bovine Serum
Recommended Host

Recommended Host:  BSC-40 (ATCC® CRL-2761)

Production host: BSC-40 (ATCC CRL-2761) Alternate hosts: MRC-5, BSC-1, HeLa, LLC-MK2
Effect on Host
Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing.
Name of Depositor S. Dales, R. Condit
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746

Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
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