Vaccinia virus ts mutant (ATCC® VR-3151)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen

Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus ts mutant
Strain IHD-W Dts56
Applications
Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox and cowpox viruses.
It was not assigned to a Dales EM category (Dales, et al, 2003), but was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D5, an ATPase required for DNA replication.
Biosafety Level 2 BSL 2
Isolation
This conditional lethal mutant, originally designated plaque isolate no. 7473, was isolated from the IHD-W strain of Vaccinia by S. Dales. The IHD-W strain was derived from an IHD-J strain of Vaccinia.
Product Format frozen
Storage Conditions -70°C or colder
Comments
This vaccinia mutant, originally designated plaque isolate no. 7473, was isolated from the IHD-W strain of Vaccinia by S. Dales, presumably by mutagenesis with nitrosoguanidine followed by replication in the presence of bromodeoxyuridine. It was not assigned to a Dales EM category (Dales, et al, 2003), but was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D5, an ATPase required for DNA replication. Shifting infection conditions from permissive to non-permissive temperature (39.5 C) results in an abrupt cessation of DNA synthesis, described as a "fast stop" phenotype. Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox and cowpox viruses.
Effect on Host
Cytopathic effects in culture include cell enlargement and rounding, followed by death and sloughing
CPE: enlargement, plaques with rounding and eventual sloughing
Growth Medium Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
Recommended Host
Production Host: BSC-40 (ATCC CRL-2761)

Recommended Host: BSC-40 (ATCC CRL-2761)

Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Growth Conditions
1-3 days at 31 C with 5% CO2
Effect on Host
Cytopathic effects in culture include cell enlargement and rounding, followed by death and sloughing
CPE: enlargement, plaques with rounding and eventual sloughing
Name of Depositor S. Dales, R. Condit
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746

Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm