Vaccinia virus (ATCC® VR-3125)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen

Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus
Strain IHD-W Dts28
Applications
The mutation was assigned by complementation analysis to vaccinia map location A24, the 132-kDa viral RNA polymerase subunit.
Biosafety Level 2 BSL 2Vaccination recommended for lab staff mipulating vaccinia, monkeypox, or cowpox viruses
Isolation
This conditional lethal mutant, originally designated plaque isolate no. 3679, was isolated from the IHD-W strain of Vaccinia by S. Dales. The IHD-W strain was derived from an IHD-J strain of Vaccinia.
Product Format frozen
Storage Conditions -70°C or colder
Comments
Selected after mutagenesis with nitrosoguanidine. The non-permissive incubation temperature is 39.5 C. This mutant was assigned a Dales EM categories G and P, characterized by incomplete membranes with spicules, immature normal particles and mature particles with cores that are predominantly lucent at the center. The mutation was assigned by complementation analysis to vaccinia map location A24, the 132-kDa viral RNA polymerase subunit.
Effect on Host
CPE: enlargement, plaques with rounding and eventual sloughing
Cytopathic effects in cell culture include cell rounding and death, with eventual sloughing
Growth Medium ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
Recommended Host
Production Host: BSC-40 (ATCC CRL-2761)

Recommended Host: BSC-40 (ATCC CRL-2761)

Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Growth Conditions
1-3 days at 31 C with 5% CO2
Effect on Host
CPE: enlargement, plaques with rounding and eventual sloughing
Cytopathic effects in cell culture include cell rounding and death, with eventual sloughing
Name of Depositor S Dales, R. Condit
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570