Human adenovirus 3 (ATCC® VR-3)

Classification: Adenoviridae, Mastadenovirus, Human adenovirus B  /  Product Format: frozen 1 mL per vial

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Classification Adenoviridae, Mastadenovirus, Human adenovirus B
Agent Human adenovirus 3
Strain GB
Applications
Respiratory research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments Agglutinates rhesus monkey red blood cells. Weakly oncogenic, contains soluble group-specific CF antigen.
Effect on Host
CPE, rounding, clumping, and sloughing
Recommended Host
A549 (ATCC® CCL-185™)
Growth Conditions
Temperature: 37°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-85% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-3 days
Effect on Host
CPE, rounding, clumping, and sloughing
Name of Depositor RJ Huebner, National Institute of Allergy and Infectious Diseases
Source Nasal washings from an adult male with a common cold, Maryland, 1953
References

Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904

Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016

Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828

Rowe WP, et al. Studies of the adenoidal-pharyngeal-conjunctival (APC) group of viruses. Am. J. Hyg. 61: 197-218, 1955.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation