Canine adenovirus 1 (ATCC® VR-293)

Classification: Adenoviridae, Mastadenovirus, Canine adenovirus A  /  Product Format: frozen 1 mL per vial

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Classification Adenoviridae, Mastadenovirus, Canine adenovirus A
Deposited As Infectious canine hepatitis virus
Agent Canine adenovirus 1
Strain Utrecht
Applications
Respiratory research
Biosafety Level 2
Isolation
Lethal infection in dog, Netherlands, 1958
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-293 Images
Effect on Host CPE, cell rounding and sloughing
Recommended Host MDCK (ATCC CCL-34)
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum.  End adsorption by adding virus growth medium.
Incubation: 3-8 days
Effect on Host CPE, cell rounding and sloughing
Name of Depositor JG Kapsenberg, Rijks Instituut Voor de Volksgezondheid, The Netherlands
Source Lethal infection in dog, Netherlands, 1958
Year of Origin 1958
References

Kapsenberg JG. Relationship of infectious canine hepatitis virus to human adenovirus. Proc. Soc. Exp. Biol. Med. 101: 611-614, 1959. PubMed: 14404448

Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828

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