Vaccinia virus (ATCC® VR-2036)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen

Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus
Strain vP-3
Biosafety Level 2
Isolation
A BamHI fragment coding for herpes TK was cloned into the BamHI site of the vaccinia HindIII F-fragment. This construct was then cloned into VTK-79 (ATCC VR-2031) by in vivo recombination.
Product Format frozen
Storage Conditions -70°C
Comments
A recombinant virus containing but not expressing the HSV-thymidine kinase gene.
Does not express the gene insert.
A BamHI fragment coding for herpes TK was cloned into the BamHI site of the vaccinia HindIII F-fragment. This construct was then cloned into VTK-79 (ATCC VR-2031) by in vivo recombination.
Effect on Host
Cytopathic effects in tissue culture
Growth Conditions
Incubation: 5 days
Effect on Host
Cytopathic effects in tissue culture
Name of Depositor E Paoletti
Special Collection Patent
References

Paoletti E, Panicali D. Modified vaccinia virus. US Patent 4,603,112 dated Jul 29 1986

Paoletti E, Panicali D. Method for immunizing animals with synthetically modified vaccinia virus. US Patent 4,722,848 dated Feb 2 1988

Panicali D, et al. Two major DNA variants present in serially propagated stocks of the WR strain of vaccinia virus. J. Virol. 37: 1000-1010, 1981. PubMed: 6262520

A BamHI fragment coding for herpes TK was cloned into the BamHI site of the vaccinia HindIII F-fragment. This construct was then cloned into VTK-79 (ATCC VR-2031) by in vivo recombination.