Koi herpesvirus (ATCC® VR-1592)

Classification: Herpesviridae, unclassified  /  Product Format: frozen

Classification Herpesviridae, unclassified
Agent Koi herpesvirus
Strain F347
Common Name Koi herpesvirus
Applications
Deposited as a useful research reagent for study of this recently identified pathogen affecting fisheries around the world.
Biosafety Level 2 BSL 2
Isolation
Isolated by K. Way, CEFAS, in England, 2004
Product Format frozen
Storage Conditions  -70°C or colder
Comments
Deposited as a useful research reagent for study of this recently identified pathogen affecting fisheries around the world. This particular isolate has not been sequenced, but several other representatives of this agent have been (Hutoran M, et al. 2005).
Effect on Host
Nephritis, gill necrosis and death in vivo. Intracellular vacuolation in infected cell culture in vitro.
Recommended Host

Recommended Host:  CCB cells (cyprinus carp brain)

Production Host: Cyprinus carp brain (CCB) cells
Growth Conditions
7-14 days at 20°C in EMEM supplemented with 10% FBS, L-glutamine and non-essential amino acids
Cryopreservation
Effect on Host
Nephritis, gill necrosis and death in vivo. Intracellular vacuolation in infected cell culture in vitro.
Name of Depositor C. Hartley
Year of Origin 2004
References

Hutoran M., et al. Description of an as yet unclassified DNA virus from diseased cyprinus carpio species. J. Virol. 79: 1983-1991, 2005. PubMed: 15681400

Bercovier, H, et al. Cloning of the koi herpesvirus (KHV) gene encoding thymidine kinase and its use for a highly sensitive PCR based diagnosis. BMC Microbiology 5: 13, 2005. PubMed: 15774009

Waltzek, TB, et al. Koi herpesvirus represents a third cyprinid herpesvirus (CyHV-3) in the family Herpesviridae. J. Gen. Virol. 86: 1659-1667, 2005. PubMed: 15914843

Gilad O., et al. Molecular comparison of isolates of an emerging fish pathogen, koi herpesvirus, and the effect of water temperature on mortality of experimentally infected koi. J. Gen. Virol. 84: 2661-2668, 2003. PubMed: 13679599