Porcine cytomegalovirus (ATCC® VR-1499)

Classification: Herpesviridae, unclassified  /  Product Format: frozen

Permits and Restrictions

View Permits

Classification Herpesviridae, unclassified
Agent Porcine cytomegalovirus
Strain Kanitz
Common Name Porcine cytomegalovirus
Biosafety Level 2
Isolation
spleen, liver, and kidney homogenate of a pig
Product Format frozen
Storage Conditions vapor phase of liquid nitrogen
Comments
Virus may be important for xenotransplantation.
Effect on Host
Yes, in vitro effects: Cytopathic effects (foci of grainy cells, some giant cells that become detached)
CPE upon co-cultivation with PTK-75 cells (CRL-2528): foci of grainy cells, some giant cells that become detached
Yes, in vivo effects: edema in lungs of pig
Yes, in vivo effects: petechiation in kidneys of pig
Recommended Host

Host of Choice: No host needed for passage.  Preparation

        consists of persistently infected cells.

Host Range: Primary porcine turbinate (PT-K75: CRL-2528) cells; Pr pig lung, Pr swine testicle, Pr porcine turbinate

Growth Conditions
Duration: 4-6 days at 37C in PTK-75 cells (ATCC CRL-2528), 5% CO2
Cryopreservation
Storage temperature: liquid nitrogen vapor phase
Effect on Host
Yes, in vitro effects: Cytopathic effects (foci of grainy cells, some giant cells that become detached)
CPE upon co-cultivation with PTK-75 cells (CRL-2528): foci of grainy cells, some giant cells that become detached
Yes, in vivo effects: edema in lungs of pig
Yes, in vivo effects: petechiation in kidneys of pig
Name of Depositor CL Kanitz
Special Collection NCRR Contract
Chain of Custody
ATCC <<--CL Kanitz<<--C. L Ecuyer
Source
spleen, liver, and kidney homogenate of a pig
References

L'Ecuyer C, Corner AH. Propagation of porcine cytomegalic inclusion disease virus in cell cultures. Preliminary report. Can. J. Comp. Med. Vet. Sci. 30: 321-326, 1966. PubMed: 4291611

The virus is distributed as primary porcine turbinate cells that are persistently infected with porcine cytomegalovirus.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation