Murid herpesvirus 4 (ATCC® VR-1465)

Classification: Herpesviridae, Rhadinovirus  /  Product Format: frozen 1 mL per vial

Classification Herpesviridae, Rhadinovirus
Deposited As Murine gammaherpesvirus 68
Agent Murid herpesvirus 4
Strain WUMS
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments GenBank: U97553
Effect on Host
CPE, cell rounding and clustering
Recommended Host
BALB/3T12-3 (ATCC® CCL-164™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-10 days
Effect on Host
CPE, cell rounding and clustering
Name of Depositor KE Weck, University of Washington School of Medicine
Special Collection NCRR Contract
Source Pool of lung, spleen, heart, liver, kidney from bank vole (Clethrionomys glareolus), Czechoslovakia, 1976
Geographical Isolation Bratislava area
References

Blaskovic D, et al. Isolation of five strains of herpesviruses from two species of free living small rodents. Acta Virol. 24: 468, 1980. PubMed: 6111210

Efstathiou S, et al. Cloning and molecular characterization of the murine herpesvirus 68 genome. J. Gen. Virol. 71: 1355-1364, 1990.

Mackett M, et al. Genetic content and preliminary transcriptional analysis of a representative region of murine gammaherpesvirus 68. J. Gen. Virol. 78: 1425-1433, 1997. PubMed: 9191940

Virgin HW, et al. Complete sequence and genomic analysis of murine gammaherpesvirus 68. J. Virol. 71: 5894-5904, 1997. PubMed: 9223479

Weck KE, et al. Murine gamma-herpesvirus 68 causes severe large-vessel arteritis in mice lacking interferon-gamma responsiveness: a new model for virus-induced vascular disease. Nat. Med. 3: 1346-1353, 1997. PubMed: 9396604

Cross References

Nucleotide (GenBank) : U97553 Murine herpesvirus 68 strain WUMS, complete genome