Perkinsus olseni (ATCC® PRA-183)

Strain Designations: Mie-13V/H4  /  Depositor: C Dungan  /  Biosafety Level: 2

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Strain Designations Mie-13V/H4
Biosafety Level 2
Isolation
marine invertebrate
Mie, Japan
Isolation date: May 29, 2002
Product Format frozen
Type Strain no
Comments
This species was isolated from visceral tissue from the Manila Clam, Venerupis ( = Tapes, Ruditapes) philippinarum.
Medium Medium 1886: Perkinsus broth medium
Growth Conditions
Temperature: 25.0°C
Max Temperature: 28.0°C
Min Temperature: 25.0°C
Duration: axenic
Protocol: Aseptically transfer 0.1 ml of the culture to 10.0 ml of fresh medium in a 25 cm2 tissue culture flask. Incubate at 25C. Transfer cultures every two weeks.
Interval: 14 days
Subcultivation
Protocol: Aseptically transfer 0.1 ml of the culture to 10.0 ml of fresh medium in a 25 cm2 tissue culture flask. Incubate at 25C. Transfer cultures every two weeks.
Interval: 14 days
Mycoplasma Indeterminate
Name of Depositor C Dungan
Special Collection NSF - Protistology
Geographical Isolation Gokasho Bay
Year of Origin May 29, 2002
References

Synonym of Perkinsus olseni Lester & Davis, 1981 and P. atlanticus Azevedo, 1989 and an update on the phylogenetic position of the genus Perkinsus.. Bull. Eur. Ass. Fish Pathol. 22: 258-265, 2002.

Dungan C F, Reece K S. In vitro propagation of two Perkinsus spp. parasites from japanese manila clams Venerupis philippinarum, and description of Perkinsus honshuensis n. sp.. J. Eukaryot. Microbiol. in press: 2006..

Cross References

Nucleotide (GenBank) : DQ516709 ITS rDNA

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Basic Documentation