Choanoeca perplexa Ellis (ATCC® 50453)

Organism: Choanoeca perplexa Ellis  /  Depositor: BS Leadbeater

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Biosafety Level 1
Isolation
Atlantic Ocean off Plymouth, England, 1977
Product Format frozen
Type Strain no
Comments
Phylogenetic analyses of SSU rRNA, LSU rRNA, tubA, and hsp-90
Medium ATCC® Medium 1361: Marine flagellate medium
Growth Conditions
Temperature: 15.0°C
Duration: grown with mixed bacteria
Protocol: ATCCNO: 50453 SPEC: Place frozen ampule in a 35C water bath directly upon its arrival. Do not allow the ampule to overheat. Agitate gently and when the ampule contents have completely thawed (approximately 2 minutes), aseptically transfer the contents to 15 ml of ATCC medium 1361 (equilibrated to 15C) in a T-25 tissue culture flask. Screw the cap on tightly and incubate at 15C. Transfer the culture every 2-3 weeks in the following manner: Rub the surface of the flask with a sterile cotton swab to detach adherent cells and aseptically transfer a 0.25 ml aliquot to 15 ml of ATCC medium 1361 in a T-25 flask.
Subcultivation
Protocol: ATCCNO: 50453 SPEC: Place frozen ampule in a 35C water bath directly upon its arrival. Do not allow the ampule to overheat. Agitate gently and when the ampule contents have completely thawed (approximately 2 minutes), aseptically transfer the contents to 15 ml of ATCC medium 1361 (equilibrated to 15C) in a T-25 tissue culture flask. Screw the cap on tightly and incubate at 15C. Transfer the culture every 2-3 weeks in the following manner: Rub the surface of the flask with a sterile cotton swab to detach adherent cells and aseptically transfer a 0.25 ml aliquot to 15 ml of ATCC medium 1361 in a T-25 flask.
Name of Depositor BS Leadbeater
Year of Origin 1977
References

Leadbeater BS. . J. Mar. Biol. Assoc. U.K. 57: 285-301, 1977.

Carr M, et al. Molecular phylogeny of choanoflagellates, the sister group to Metazoa. Proc. Natl. Acad. Sci. USA. 105: 16641-16646, 2008. PubMed: 18922774

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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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