Trypanosoma sp. (ATCC® 50295)

Organism: Trypanosoma sp.  /  Depositor: LS Diamond

Strain Designations 465, clone BA
Application
Vector borne research
Biosafety Level 1
Isolation
Rana sphenocephala, FL, 1950
Product Format frozen
Type Strain no
Comments
Phylogenetic relationships among anuran trypanosomes
Species compared using RAPDs
Karyotype analysis of anuran trypanosomes
Medium ATCC® Medium 1011: Diphasic blood agar medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor LS Diamond
Year of Origin 1950
References

Clark CG, et al. Phylogenetic relationship among anuran Trypanosoma as revealed by riboprinting. J. Eukaryot. Microbiol. 42: 92-96, 1995. PubMed: 7728144

Lun ZR, Desser SS. Analysis of isolates within species of anuran trypanosomes using random amplified polymorphic DNA. Parasitol. Res. 82: 22-27, 1996. PubMed: 8825440

Lun ZR, Desser SS. Karyotype analysis of anuran trypanosomes by pulsed-field gradient gel electrophoresis. J. Parasitol. 81: 1018-1020, 1995. PubMed: 8544044