Trypanosoma neveulemairei Brumpt (ATCC® 30641)

Depositor: FG Wallace  /  Biosafety Level: 1

Permits and Restrictions

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Application
Vector borne research
Biosafety Level 1
Isolation
frog, Rana esculenta (imported), Yugoslavia (Univ. Minnesota), 1975
Product Format frozen
Type Strain no
Comments
Phylogenetic relationships among anuran trypanosomes
Karyotype analysis of anuran trypanosomes
Medium Medium 947: L diphasic blood agar (ATCC Medium 1011) with Locke's solution overlay reduced from 3.0 ml to 1.0 ml
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor FG Wallace
Year of Origin 1975
References

Clark CG, et al. Phylogenetic relationship among anuran Trypanosoma as revealed by riboprinting. J. Eukaryot. Microbiol. 42: 92-96, 1995. PubMed: 7728144

Wallace FG. What is Trypanosoma rotatorium?. J. Protozool. 22: 18A 35, 1975.

Lun ZR, Desser SS. Karyotype analysis of anuran trypanosomes by pulsed-field gradient gel electrophoresis. J. Parasitol. 81: 1018-1020, 1995. PubMed: 8544044

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation