Herpetomonas megaseliae Daggett et al. (ATCC® 30209)

Organism: Herpetomonas megaseliae Daggett et al.  /  Depositor: J Janovy

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Application
Phylogenetic analysis of RNA editing
Biosafety Level 1
Product Format frozen
Type Strain yes
Comments
species description
Multiplication in tomatoes
Distribution of carbohydrate epitopes
Monoclonal antibodies for identification
Physiological alterations accompanying infectivity
Comparative physiology
Phylogenetic analysis of RNA editing
Proteolytic activities
Acetylornithinase and ornithine acetyltransferase
Growth on blood-agar plates
Effects of hydroxyurea on morphology and growth
Cyclopropane fatty acid
Ultrastructural observations
Unique no-branched polyunsaturated fatty acids
isoleucine requirement and threonine deaminase
endonuclease-generated fragments of K-DNA, esterase isoenzymes, surface proteins for species identification
Medium ATCC® Medium 1011: Diphasic blood agar medium
ATCC® Medium 1011: Diphasic blood agar medium
ATCC® Medium 706: M medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor J Janovy
References

Yoshida N. Herpetomonas mariadeanei sp. n. (Protozoa, Trypanosomatidae) from Muscina stabulans (Fallen, 1816) (Diptera, Muscidae). J. Protozool. 25: 421-425, 1978.

Conchon I, et al. Trypanosomatids, other than Phytomonas spp., isolated and cultured from fruit. J. Protozool. 36: 412-414, 1989.

Gazzinelli RT, et al. Distribution of carbohydrates recognized by the lectins Euonymus europaeus and concanavalin A in monoxenic and heteroxenic trypanosomatids. J. Protozool. 38: 320-325, 1991. PubMed: 1787421

Faria e Silva PM, et al. Herpetomonas roitmani (Fiorini et al., 1989) n. comb.: a trypanosomatid with a bacterium-like endosymbiont in the cytoplasm. J. Protozool. 38: 489-494, 1991. PubMed: 1920148

Daggett PM. Herpetomonas megaseliae sp. n. (Protozoa: Trypanosomatidae) from megaselia scalaris (Loew, 1866) Schmitz, 1929 (Diptera: Phoridae)*. J. Parasitol. 58: 946-949, 1972.

Camargo EP, et al. Proteolytic activities in cell extracts of trypanosomatids. J. Parasitol. 64: 1120-1121, 1978. PubMed: 739304

Teixeira MM, Camargo EP. Monoclonal antibodies for the identification of trypanosomatids of the genus Phytomonas. J. Protozool. 36: 262-264, 1989.

Teixeira MM, et al. Characterization of the target antigens of Phytomonas-specific monoclonal antibodies. J. Eukaryot. Microbiol. 42: 232-237, 1995.

Daggett PM, Decker JE. Some physiological alterations accompanying infectivity to mammals by four genera of Trypanosomatidae. Comp. Biochem. Physiol. 59: 363-366, 1978.

Janovy J Jr.. Problems in the comparative physiology of some trypanosomatid flagellates. Acta Trop. 34: 177-184, 1977. PubMed: 19960

Landweber LF, Gilbert W. Phylogenetic analysis of RNA editing: a primitive genetic phenomenon. Proc. Natl. Acad. Sci. USA 91: 918-921, 1994. PubMed: 8302867

Galinari S, Camargo EP. Trypanosomatid protozoa: survey of acetylornithinase and ornithine acetyltransferase. Exp. Parasitol. 46: 277-282, 1978. PubMed: 569594

Keppel AD, Janovy J J. Herpetomonas megaseliae and Crithidia harmosa: growth on blood-agar plates. J. Parasitol. 63: 879-882, 1977. PubMed: 562401

Knight SA. Differentiation of Herpetomonas megaseliae: effects of hydroxyurea on morphology and growth. J. Parasitol. 62: 515-522, 1976. PubMed: 957031

Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247

Janovy J J, et al. The differentiation of Herpetomonas megaseliae: ultrastructural observations. J. Protozool. 21: 53-59, 1974. PubMed: 4817982

Fish WR, et al. Some Phytomonas and Herpetomonas species form unique iso-branched polyunsaturated fatty acids. Mol. Biochem. Parasitol. 5: 1-18, 1982. PubMed: 7062937

Alfieri SC, Camargo EP. Trypanosomatidae: isoleucine requirement and threonine deaminase in species with and without endosymbionts. Exp. Parasitol. 53: 371-380, 1982. PubMed: 6806116

Camargo EP, et al. Electrophoretic analysis of endonuclease-generated fragments of k-DNA, of esterase isoenzymes, and of surface proteins as aids for species identification of insect trypanosomatids. J. Protozool. 29: 251-258, 1982. PubMed: 6284925

Landweber LF, Gilbert W. RNA editing as a source of genetic variation. Nature 363: 179-182, 1993. PubMed: 8387160

type strain

Cross References

Nucleotide (GenBank) : U01006 Herpetomonas megaseliae kinetoplast 9S rRNA gene.

Nucleotide (GenBank) : U01010 Herpetomonas megaseliae kinetoplast 12S rRNA gene.

Nucleotide (GenBank) : U01014 Herpetomonas megaseliae 16S-like small subunit rRNA.

Nucleotide (GenBank) : L10848 kinetoplast cytochrome oxidase subunit III (COIII) mRNA, complete coding sequence

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