Plasmodium inui Halberstaedter and von Prowazek (ATCC® 30195)

Strain Designations: IC (Leucophyrus)  /  Depositor: National Institutes of Health  /  Biosafety Level: 2

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Strain Designations IC (Leucophyrus)
Application
Vector borne research
Biosafety Level 2
Isolation
Anopheles leucophyrus, Negri Sembilan, West Malaysia, 1964.
Product Format frozen
Type Strain no
Comments
transmission by anopheline mosquitoes
taxonomy, morphology, life cycle, and geographical distribution
phylogeny
serologic relationship of Asian isolates
Growth Conditions
Duration: in vivo, Macaca mulatta
Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Subcultivation
Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Name of Depositor National Institutes of Health
Year of Origin 1964
References

Collins WE, et al. Plasmodium inui: serologic relationships of Asian isolates. Exp. Parasitol. 27: 507-515, 1970. PubMed: 4986810

Eyles DE. The species of simian malaria: taxonomy, morphology, life cycle, and geographical distribution of the monkey species. J. Parasitol. 49: 866-887, 1963. PubMed: 14084190

Collins WE, et al. Some observations on the transmission of Plasmodium inui. J. Parasitol. 54: 846-847, 1968. PubMed: 4990409

Kissinger JC, et al. Plasmodium inui is not closely related to other quartan Plasmodium species. J. Parasitol. 84: 278-282, 1998. PubMed: 9576499

Sullivan JS, et al. Sporozoite transmission of three strains of Plasmodium knowlesi to Aotus and Saimiri monkeys. J. Parasitol. 82: 268-271, 1996. PubMed: 8604095

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