Plasmodium hylobati Rhodain (ATCC® 30154)

Strain Designations: WAK  /  Depositor: National Institutes of Health  /  Biosafety Level: 2

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Strain Designations WAK
Application
Vector borne research
Biosafety Level 2
Isolation
Hylobates molock, Sarawak, 1968
Product Format frozen
Type Strain no
Growth Conditions
Duration: in vivo, Macaca mulatta
Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Subcultivation
Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Name of Depositor National Institutes of Health
Year of Origin 1968
References

Collins WE, et al. Plasmodium hylobati: a malaria parasite of the gibbon. J. Parasitol. 58: 123-128, 1972. PubMed: 4335047

Coatney GR. The primate malarias. Washington, DC: Government Printing Office; 1971.

Sodeman TM, et al. Studies of the exoerythrocytic stages of simian malaria. VI. Plasmodium hylobati. J. Parasitol. 58: 129-134, 1972. PubMed: 4335048

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