Primary Small Airway Epithelial Cells; Normal, Human (ATCC® PCS-301-010)

Organism: Homo sapiens, human  / 

Permits and Restrictions

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Organism Homo sapiens, human
Morphology Epithelial, packed cuboidal morphology
Growth Properties Adherent
Biosafety Level 1
Age Batch-specific
Gender Batch-specific
Ethnicity Batch-specific
Applications
Microbial (e.g., viral, bacterial) infection and pathogenesis; airway inflammation and wound healing; asthma; pulmonary fibrosis, chronic obstructive pulmonary disease; emphysema; toxicology/other testing of pharmaceuticals.
Product Format frozen 1 mL
Storage Conditions -130°C or below
Comments
Human Bronchial Tracheal Smooth Muscle Cells are cryopreserved at passage 2 (cells have been isolated, plated, and expanded in culture vessels twice prior to cryopreservation) to ensure the highest viability and proliferation efficiency. These cells, when transiently transfected using TransfeX Transfection Reagent (ATCC® ACS-4005™), express high levels of GFP.
Vascular Cell Basal Medium (ATCC PCS-100-030), when supplemented with Vascular Smooth Muscle Cell Growth Kit (ATCC PCS-100-042), provides an ideal system to propagate bronchial tracheal smooth muscle cells.
Complete Growth Medium
    1. Obtain one Small Airway Epithelial Cell Growth Kit from the freezer; make sure that the caps of all components are tight.
    2. Thaw the components of the growth kit just prior to adding them to the basal medium.
    3. Obtain one bottle of Airway Epithelial Cell Basal Medium (485 mL) from cold storage.
    4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
    5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component, as indicated in Table 1, to the bottle of basal medium using a separate sterile pipette for each transfer.
    6. Antimicrobials and phenol red are not required for proliferation but may be added if desired. The recommended volume of each optional component to be added to the complete growth media is summarized in Table 2.
    7. Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle.
    8. Complete growth media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete growth media is stable for 30 days.

 Table 1. Brochial/Tracheal Epithelial Cell Growth Kit Components

Component

Volume

Final Concentration

HLL Supplement

1.25 mL

HSA 500 mg/mL

Linoleic Acid 0.6 mM

Lecithin 0.6 mg/mL

L-Glutamine

15 mL

6 mM

Extract P

2.0 mL

0.4%

Airway Epithelial Cell Supplement

5.0 mL

Epinephrine 1.0 mM

Transferrin 5 mg/mL

T3 10 nM

Hydrocortisone 5 mg/mL

rh EGF 5 ng/mL

rh Insulin 5 mg/mL

Antimicrobials and phenol red are not required for proliferation but may be added if desired. The recommended volume of each optional component to be added to the complete growth media is summarized in Table 2.

 

Table 2. Addition of Antimicrobials/Antimycotics and Phenol Red (Optional)

Component

Volume

Final Concentration

Gentamicin-Amphotericin B Solution

0.5 mL

Gentamicin: 10 µg/mL

Amphotericin B: 0.25 µg/mL

Penicillin-Streptomycin-Amphotericin B Solution

0.5 mL

Penicillin: 10 Units/mL

Streptomycin: 10 µg/mL

Amphotericin B: 25 ng/mL

Phenol Red

0.5 mL

33 µM

  1. Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle.
  2. Complete growth media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete growth media is stable for 30 days.
Volume 1 mL
Sterility Tests
Bacteria and Yeast: Negative
Mycoplasma: Negative
Viral Testing
Hepatitis B: Negative
Hepatitis C: Negative
HIV: Negative
Viability ≥ 50% when thawed from cryopreservation.
Population Doubling Time ≥ 15 in complete growth medium
C of A
Certificate of Analysis
Certificate of Analysis
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Aramwit P, et al. An anti-cancer cordycepin produced by cordyceps militaris growing on the dead larva of bombyx mori silkworm. J. Agr. Sci .6(6): 41, 2014.