Primary Cells

  • Fibroblast Growth Kit-Serum-free (ATCC® PCS-201-040™)

    ATCC® Number: PCS-201-040 ™

    For-Profit:   $79.00 Non-Profit:   $79.00

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    Product Format: frozen 1 kit

  • Primary Pulmonary Artery Smooth Muscle Cells; Normal, Human (ATCC® PCS-100-023™)

    ATCC® Number: PCS-100-023 ™
    Organism: Homo sapiens, human
    Cell Type: smooth muscle
    Tissue: artery

    For-Profit:   $629.00 Non-Profit:   $629.00

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    Disease: normal
    Product Format: frozen 1 mL

  • Fibroblast Basal Medium (ATCC® PCS-201-030™)

    ATCC® Number: PCS-201-030 ™
    Quantity: 480 ml
    Storage: 2 to 8°C; protect from light
    Applications:

    Fibroblast Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This system is designed to support fibroblasts derived from normal human epidermis. Fibroblast Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts.

    1. To support the proliferation and plating efficiency of fibroblasts (e.g., Dermal Fibroblasts, Normal, Human Neonatal [ATCC PCS-201-010], Dermal Fibroblasts, Normal, Human Neonatal, Mitomycin C Treated [ATCC PCS-201-010]), Fibroblast Basal Medium must be supplemented with one of the following kits:
      1. Fibroblast Growth Kit–Serum-Free (ATCC PCS-201-040)
      2. Fibroblast Growth Kit–Low Serum (ATCC PCS-201-041)
    2. Optional media supplements:
      1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
      2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
      3. Phenol Red (ATCC PCS-999-001)

    For-Profit:   $49.00 Non-Profit:   $49.00

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    Product Format: 480 ml

  • GeneXPlus Transfection Reagent (ATCC® ACS-4004™)

    ATCC® Number: ACS-4004 ™
    Quantity: 1 mL
    Storage: -20°C

    For-Profit:   $350.00 Non-Profit:   $350.00

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    Product Format: 1 mL
    Comments:

    This reagent affords high levels of gene expression in a variety of cell types and is suitable for both transient and stable transfection. GeneXPlus works effectively in large scale reactions, producing high protein yields. Since the reagent is composed of animal-origin free components and is serum compatible, there is no need for any culture medium change after transfection.

     

    GeneXPlus Transfection Reagent has been optimized for use with HEK293T/17 SF suspension cells (ATCC ACS-4500) and HEKPlus SFM (ATCC ACS-4004) to reproducibly express a wide range of proteins at high levels.

  • Dermal Cell Basal Medium (ATCC® PCS-200-030™)

    ATCC® Number: PCS-200-030 ™
    Quantity: 485 ml
    Storage: 2 to 8°C, protect from light
    Applications: Dermal Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC Primary Cell Solutions™ system.

    This system is designed to support cells (e.g., keratinocytes, melanocytes) derived from normal human epidermis. Dermal Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. 

    To support the proliferation and plating efficiency of keratinocytes (e.g., Primary Epidermal Keratinocytes, Normal, Human Neonatal Foreskin, ATCC PCS-200-010), Dermal Cell Basal Medium must be supplemented with the Keratinocyte Growth Kit (ATCC PCS-200-040). The complete serum-free (although not animal-free) media is formulated to inhibit fibroblast growth, and the low calcium concentration (0.06 mM) slows differentiation.

    To support the proliferation and plating efficiency of melanocytes (e.g., Primary Epidermal Melanocytes, Normal, Human Neonatal, ATCC PCS-200-012 or Primary Epidermal Melanocytes, Normal, Human Adult, ATCC PCS-200-013), Dermal Cell Basal Medium must be supplemented with either the Melanocyte Growth Kit (ATCC PCS-200-041) or the Adult Melanocyte Growth Kit (ATCC PCS-200-042).

    Using Dermal Cell Basal Medium supplemented with the appropriate growth kit, the growth of keratinocytes and melanocytes are supported without the use of feeder layers, extracellular matrix proteins or other substrates.

    Optional media supplements:

    1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
    2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    3. Phenol Red (ATCC PCS-999-001)

     

    For-Profit:   $49.00 Non-Profit:   $49.00

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    Product Format: 485 ml

  • (ATCC® CRL-4002™)

    ATCC® Number: CRL-4002™
    Notice: The ATCC number you are searching for is currently not available; it may be in production. You can e-mail Customer Service with the ATCC number to check the status of the item.

    Notice: Product Unavailable

  • 0.1% Gelatin Solution (ATCC® PCS-999-027™)

    ATCC® Number: PCS-999-027 ™

    For-Profit:   $27.00 Non-Profit:   $27.00

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    Product Format: 100 ml

  • Primary Epidermal Melanoyctes; Normal, Human, Neonatal (ATCC® PCS-200-012™)

    ATCC® Number: PCS-200-012 ™
    Organism: Homo sapiens, human
    Cell Type: melanocyte
    Tissue: foreskin

    For-Profit:   $479.00 Non-Profit:   $479.00

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    Disease: normal
    Product Format: frozen 1 mL

  • VeroPlus SFM (ATCC® ACS-4001™)

    ATCC® Number: ACS-4001 ™
    Quantity: 500 mL
    Storage: Store product at 2°C to 8°C. Do not freeze.

    For-Profit:   $40.00 Non-Profit:   $40.00

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    Product Format: 500 mL
    Comments:

    Each lot of VeroPlus SFM is tested functionally for growth characteristics and morphology with Vero cells. Additionally, the media is assayed for pH, osmolality, endotoxins and sterility.

    Supplementing with L- Glutamine or L-alanyl-L-Glutamine is required before use.

  • HEKPlus Protein Expression System (ATCC® ACS-4800-K™)

    ATCC® Number: ACS-4800-K ™
    Applications:

    HEKPlus Expression System is a complete cell culture system consisting of suspension cells, xeno-free, serum-free medium, transfection reagent and boost reagent that is optimized for transient transfection and protein expression. The components have been demonstrated to work together to reproducibly express a wide range of proteins at high levels. HEK293T/17 SF cells are pre-adapted to HEKPlus SFM and can be cultured in suspension and directly transfected in HEKPlus SFM using GeneXPlus transfection reagent. Transfection complexes can be prepared in the HEKPlus medium as well. Boost reagent has been shown to significantly enhance extracellular protein expression levels in cultures at volumes greater than 5 mL.

    The HEKPlus Expression System supplies sufficient reagents for the transfection of the equivalent of 500 mL of culture or 1 x 109 total cells (e.g. 16 x 30 mL reactions, 50 x 10 mL reactions) at an optimized transfection reagent ratio of 1:2 for DNA (µg) to transfection reagent (µL). The reagent volumes can be easily scaled up, if large-scale protein production is desired.

    For-Profit:   $740.00 Non-Profit:   $740.00

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