Streptomyces griseus subsp. griseus (Krainsky) Waksman and Henrici (ATCC® 10137)

Strain Designations: 4 [BUCSAV 7, Boots FD40, NCIB 8232, NCTC 6961]  /  Type Strain: no  /  Biosafety Level: 1

Strain Designations 4 [BUCSAV 7, Boots FD40, NCIB 8232, NCTC 6961]
Produces cytochrome P450 and glutaminase.
Transformation of pergolide to pergolide sulfoxide and of 1',2'-dihydrorotenone.
Derived from existing strain
Type Strain no
Biosafety Level 1
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® yes
Type Strain no
Medium ATCC® Medium 1877: ISP Medium 1
ATCC® Medium 196: Yeast malt extract agar
Growth Conditions
Temperature: 26°C
Atmosphere: Aerobic
Name of Depositor SA Waksman
Derived from existing strain

Sariaslani FS, Rosazza JP. Biotransformations of 1',2'-dihydrorotenone by Streptomyces griseus. Appl. Environ. Microbiol. 49: 451-452, 1985. PubMed: 3920966

Yokotsuka T, et al. Hydrolysis of glutamine within foods and beverages to glutamic acid. US Patent 3,717,470 dated Feb 20 1973

Proc. Soc. Exp. Biol. Med. 55: 66-69, 1944.

Kunz DA, Sariaslani SF. Process for inducing cytochrome P-450 enzymes in Streptomyces bacteria and determining the mutagenicity of chemicals. US Patent 5,674,714 dated Oct 7 1997

Smith RV, et al. Microbial transformations of pergolide to pergolide sulfoxide and pergolide sulfone. J. Pharm. Sci. 72: 733-736, 1983. PubMed: 6684155

derived from Waksman's original streptomycin-producing strain 3463

Cross References

Nucleotide (GenBank) : X17368 S. griseus 5S rRNA.

Nucleotide (GenBank) : X55551 S.griseus saf gene.

Nucleotide (GenBank) : X74868 S.griseus gene for glutamic acid specific protease.

Nucleotide (GenBank) : M64471 Streptomyces griseus (ATCC 10137) trypsin (sprT) gene, complete cds.