pEM304 [pHUMDUT2] (ATCC® 79039)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations pEM304 [pHUMDUT2]
GenBank Number

M89913

Species Homo sapiens, human
Applications
in another host, produces protein dUTP pyrophosphatase
Vector
Construct size (kb): 3.700000047683716
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTZ19R
Intact vector size: 2.900
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC19, Escherichia coli lacZalpha, pBR322, f1
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DUT*
Genomic copy number: multiple
Gene name: dUTP pyrophosphatase (dUTPase)
Contains complete coding sequence?: Y
Chromosome: 15; Localization: 15, cR
Type of DNA: cDNA
Insert end: Modification: EcoRI adapters
Insert end: Modification: EcoRI adapters
Insert size (kb): 0.8
Cross references: DNA Seq. Acc.: M89913
Insert lengths(kb): 0.800000011920929
Gene product: dUTP pyrophosphatase [DUT*]
Target Gene: dUTP pyrophosphatase
Insert Size (kb) 0.800
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 0.8; HindIII--3.7; XmnI--2.2, 1.4, 0.2; SmaI--3.0, 0.7; SphI--2.95, 0.75.
The insert contains the following restriction sites (nt from the 5' end): SphI--43; SmaI--91, XmnI--653.
Contains the complete coding sequence, and corresponds to nt 43-837 of the database record.
The antisense strand is packaged.
pEM303 (ATCC 79036/7) and pEM304 (ATCC 79038/9) differ only in the orientation of the insert.
References

McIntosh EM, et al. Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme. Proc. Natl. Acad. Sci. USA 89: 8020-8024, 1992. PubMed: 1325640

Evan M McIntosh, personal communication

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