2Q1 (ATCC® 77419)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: F Kao

Permits and Restrictions

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Designations 2Q1
Species Homo sapiens, human
Depositors F Kao
Origin
lymphoblast, normal, female (GM03714)
Applications
Microdissection library derived from human chromosome 2q35-37. Can be used for chromosome painting and for isolating probes specific for the 2q35-37 region.
Vector
Tissue: lymphoblast, normal, female (GM03714)
Chromosome: 2q35-q37
DNA: genomic
Digest: Complete
Insert size range: 0.05 - 0.8 kb
Genome Equivalent: 0.0
Coverage: Clones for "complete" coverage: 20000.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
Microdissection library derived from human chromosome 2q35-37. Can be used for chromosome painting and for isolating probes specific for the 2q35-37 region.
Deposited as PCR products. These were digested with MboI before cloning.
Approximately 25% of the inserts are non-human.
The library has about 40-50% repetitive sequence clones which are human specific.
References

Yu J, et al. Construction and characterization of a region-specific microdissection library from human chromosome 2q35-q37. Genomics 14: 769-774, 1992. PubMed: 1427905

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