AMJIA65 (ATCC® 626280)

Organism: Methanocaldococcus jannaschii (Jones et al.) Whitman  /  Clone Type: Micro  /  Depositors: The Institute for Genomic Research

Permits and Restrictions

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Designations AMJIA65
GenBank Number

L77117

Species Methanocaldococcus jannaschii (Jones et al.) Whitman
Depositors The Institute for Genomic Research
Vector
Construct size (kb): 4.833000183105469
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: plasmid
Vector end: SmaI
Vector end: SmaI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Methanococcus jannaschii
Gene name: biotin synthetase
ORF's seq. position: DNA Seq. Acc.: L77117 (1244593-1243520)
Contains complete coding sequence?: Y
Type of DNA: genomic
Insert end: Modification: blunted
Insert end: Modification: blunted
Insert size (kb): 2.147
Cross references: DNA Seq. Acc.: L77117
Nucleotides 1-2147 of the insert correspond to
nucleotides 1242581-1244727 of L77117.
Insert lengths(kb): 2.147000074386597
Gene product: biotin synthetase
Insert Size (kb) 2.147
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
References

Bult CJ, et al. Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii. Science 273: 1058-1073, 1996. PubMed: 8688087

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