pEco63 [clone of hepatitis B] in E. coli (ATCC® 31518)

Organism: Hepatitis B virus, Hepatitis B virus  /  Clone Type: Clone  /  Depositors: Regents of the University of California

Permits and Restrictions

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Designations pEco63 [clone of hepatitis B] in E. coli
Species Hepatitis B virus, Hepatitis B virus
Depositors Regents of the University of California
Applications
Blood borne research
Sexually Transmitted Disease Research
Vector
Construct size (kb): 9.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 3.200000047683716
Insert Size (kb) 3.200
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2
Shipping Information

Shipped:  Frozen glycerol stock of E. coli containing the plasmid

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.1, 3.5; HindIII--10.
Full-length clone (insert size 3.2 kb). This material is cited in a U.S. and/or other patent Application and may not be used to infringe the patent claims.
Constructed by inserting a full-length genome of hepatitis B cut with EcoRI into EcoRI site of pBR325
Constructed by inserting a full-length genome of hepatitis B virus cut with EcoRI into the EcoRI site of pBR325.
Classification Hepadnaviridae, Orthohepadnavirus
References

Chow YH, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2. J. Virol. 71: 169-178, 1997. PubMed: 8985336

Kate H Murashige, personal communication

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