Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs - Low Serum (ATCC® PCS-500-040)

Biosafety Level 1
Product Format frozen 1 Kit
Storage Conditions -20°C (or -70°C for long-term storage)
Comments
Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs - Low Serum contains components that when added to Mesenchymal Stem Cell Basal Medium (ATCC PCS-500-030) create a complete cell culture environment for mesenchymal stem cells isolated from normal human adipose and umbilical tissue. The final concentration for each component in complete mesenchymal stem cell growth medium is as follows:
  • rh FGF basic: 5 ng/mL
  • rh FGF acidic: 5 ng/mL
  • rh EGF: 5 ng/mL
  • Fetal Bovine Serum: 2%
  • L-Alanyl-L-Glutamine: 2.4 mM
Complete Growth Medium
  1. Obtain one Mesenchymal Stem Cell Growth Kit– for Adipose and Umbilical-derived MSCs - Low serum from the freezer; make sure that the caps of all components are tight.
  2. Thaw the components of the growth kit just prior to adding them to the basal medium.
  3. Obtain one bottle of Mesenchymal Stem Cell Basal Medium (485 mL) from cold storage.
  4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
  5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component, as indicated in Table 1, to the bottle of basal medium using a separate sterile pipette for each transfer.
  6. Table 1. Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs - Low Serum Components

    Component                     

    Volume

    Final Concentration

    MSC Supplement

    10 mL

    2% FBS

    5 ng/mL rh FGF basic

    5 ng/mL rh FGF acidic

    5 ng/mL rh EGF

    L-Alanyl-L-Glutamine

    6 mL

    2.4 mM

     

    Antimicrobials and phenol red are not required for proliferation but may be added if desired. The recommended volume of each optional component to be added to the complete growth media is summarized in Table 2.

     

    Table 2. Addition of Antimicrobials/Antimycotics and Phenol Red (Optional)

    Component

    Volume

    Final Concentration

    Gentamicin-Amphotericin B Solution

    0.5 mL

    Gentamicin: 10 µg/mL

    Amphotericin B: 0.25 µg/mL

    Penicillin-Streptomycin-Amphotericin B Solution

    0.5 mL

    Penicillin: 10 Units/mL

    Streptomycin: 10 µg/mL

    Amphotericin B: 25 ng/mL

    Phenol Red

    0.5 mL

    33 µM


  7. Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle.
  8. Complete growth media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete growth media is stable for two weeks. 
Volume 1 Kit
Sterility Tests
Negative for bacteria, fungi and yeast
Functional Tests Rate of proliferation and morphology
C of A
Certificate of Analysis
Certificate of Analysis