Trypsin-EDTA Solution, 1X (ATCC® 30-2101)

Product Format frozen 100 mL
Storage Conditions Store Trypsin-EDTA solution frozen, between -5°C and -20°C. Avoid repeated freeze-thaws by dispensing and storing in aliquots.
Applications
0.25% Trypsin/0.53 mM EDTA in Hanks Balanced Salt Solution without calcium or magnesium. For dissociation of cell monolayers. Trypsin-EDTA solution is suitable for most but not for all adherent cell lines. For cell line-specific information, please go to the appropriate product page on the web, refer to the product sheet supplied with the cell line, or contact ATCC Technical Service.
Comments NOTE:  Each cell line responds to ATCC® Trypsin-EDTA solution in a unique manner.  For optimum results, observe the cells during the dissociation process to avoid over-trypsinization.  For more information, please refer to the General Protocol for Using ATCC® Trypsin-EDTA Solution.  For cell line-specific information, please refer to the product sheet supplied with the cell line, or contact ATCC® Technical Service. ATCC® Trypsin-EDTA solution is suitable for most but not for all adherent cell lines. This product contains phenol red.
Biosafety Level N/A
Quality Control Specifications

Lot specific results are available in the Certificate of Analysis, which is available at www.atcc.org or by contacting ATCC technical service.

SPECIFICATION CERTIFICATE

Test

Specification

pH

7.0 to 8.0

Osmolality

283 to 313 mOsm/kg

Sterility Testing

Pass

Cell Culture Tests – MRC-5 (CCL-171)

Pass

Cell Culture Tests – Vero (CCL-81)

Pass

Porcine Parvovirus (PPV)

Not Detected

Mycoplasma

Not Detected

*Please consult the Certificate of Analysis for lot-specific test results.