Media Sera Reagents

  • Hank's Balanced Salt Solution (HBSS), 1X (ATCC® 30-2213)

    ATCC® Number: 30-2213
    Quantity: 500 mL
    Storage: Store Hank's BSS at ambient temperatures, between 15°C and 30°C
    Applications: For washing cells, for use as a diluent, or for use as an inorganic base for standard media preparation.

    For-Profit:   $14.00 Non-Profit:   $14.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    Contains no calcium, magnesium, or phenol red.
  • Fetal Bovine Serum, ES Cell Qualified (ATCC® SCRR-30-2020)

    ATCC® Number: SCRR-30-2020
    Quantity: 500 mL
    Storage: Store fetal bovine serum at -70°C in its yellow plastic storage bag. The yellow bag minimizes the effect of light on the serum. If storage at -70°C is not possible, then store at -20°C. Do not store fetal bovine serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing fetal bovine serum in aliquots. Fetal bovine serum is supplied in PETG plastic bottles.
    Applications:

    This serum is tested for ability to support the growth and maintenance of embryonic stem cells in an undifferentiated state. Undifferentiated embryonic stem cells are determined by colony morphology and expression of three pluripotent markers. The results are compared to a control lot of serum. The serum is triple filtered through 0.1µM filters and each lot of fetal bovine serum is tested for sterility. Fetal bovine serum is manufactured from fetal bovine blood collected in USDA inspected abattoirs located in the United States.


    For-Profit:   $424.00 Non-Profit:   $424.00

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    Product Format: frozen 500 mL
    Comments: The results were compared with cells grown with a control lot of serum.
  • TSB with 10% Glycerol (ATCC® 20-2200)

    ATCC® Number: 20-2200
    Quantity: Package of 12 (1.0 mL/ vial)
    Storage: Store at 4 to 8°C
    Applications:

    Trypticase soy broth (TSB) with 10% glycerol (v/v) is used as a freeze medium for storage of nonfastidious bacteria, fungi, and yeast. Product is provided in an individual unit of use (1.0 mL).

    For-Profit:   $65.00 Non-Profit:   $65.00

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  • Mouse ES Cell Basal Medium (ATCC® SCRR-2011)

    ATCC® Number: SCRR-2011
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: General culture conditions to maintain undifferentiated,  pluripotent mouse ES cells are now well established, and  incremental improvements, such as the development of  Mouse ES Cell Basal Medium, are key to the ongoing  success of stem cell research.

    Mouse ES Cell Basal Medium is a sterile liquid tissue culture  medium containing phenol red that has been optimized to  support the undifferentiated self-renewal cycle related to  mouse embryonic stem cell growth.

    The medium contains a stable glutamine dipeptide, providing  a long shelf-life when handled and stored properly medium may be supplemented by addition of ES-Cell  Qualified Fetal Bovine Serum (FBS) or a serum replacement  designed for mouse ES cells. This medium can support the growth of mouse ES cells on  either a mitotically inactivated layer of mouse embryonic  fibroblast (MEF) feeder cells, or in a feeder-free environment using gelatin-coated plates.

    For-Profit:   $25.00 Non-Profit:   $25.00

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  • Phosphate Buffered Saline Without Calcium Or Magnesium (ATCC® SCRR-2201)

    ATCC® Number: SCRR-2201
    Quantity: 500 mL
    Storage: Store at ambient temperatures, between 15°C and 30°C.
    Applications:

    For washing cells (including embryonic stem cells), for use as a diluent, or for use as an inorganic base for standard media preparation.

    For-Profit:   $13.00 Non-Profit:   $13.00

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  • CellMatrix Basement Membrane Gel (ATCC® ACS-3035)

    ATCC® Number: ACS-3035
    Quantity: 5 mL
    Storage: -80°C or colder

    For-Profit:   $205.00 Non-Profit:   $205.00

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    Product Format: frozen 5 mL
    Comments:
    Each lot of CellMatrix Gel is shown to promote the attachment of hESCs/hiPSCs and to maintain hESCs/hiPSCs in a pluripotent state as determined by the expression of Oct-4 and Nanog pluripotent markers.
    Store at -80°C in aliquots. Avoid repeated freezing and thawing.
  • Hybri-Care Medium (ATCC® 46-X)

    ATCC® Number: 46-X
    Quantity: 1 Package (prepares 1L of medium)
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Hybri-Care medium is based on the formulation used by D.H. Sachs and collaborators for propagation of hybridomas and other fastidious cell lines.
    A combined buffering system of HEPES and added NaHCO3 enables the medium to be used at all stages of hybridoma production from fusion to cloning, including single-cell subcloning.

    For-Profit:   $18.00 Non-Profit:   $18.00

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    Formulation: Formulation
    Product Format: 1 Package (prepares 1L of medium)
    Comments:
    Hybri-Care medium is based on the formulation used by D.H. Sachs and collaborators for propagation of hybridomas and other fastidious cell lines. Ingredients are essentially those of a modified DMEM and NCTC 135 plus insulin. A combined buffering system of HEPES and added NaHCO3 enables the medium to be used at all stages of hybridoma production from fusion to cloning, including single-cell subcloning.

    When used with 10% fetal bovine serum, Hybri-Care repeatedly has been found to support vigorous growth of most hybridomas in the ATCC collection.

    Ozato, K., et al. J. Immunology 124: 533-540, 1980.
  • Calf Bovine Serum (ATCC® 30-2031)

    ATCC® Number: 30-2031
    Quantity: 100 mL
    Storage: Store calf bovine serum at -20°C or colder. Do not store frozen calf bovine serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing calf bovine serum in aliquots. Calf bovine serum is supplied in PETG plastic bottles.
    Applications: Iron supplemented and triple filtered through 0.1 μm filters. Each lot of calf bovine serum is tested for sterility and for the ability to support the growth of cells using sequential growth curves. Calf bovine serum is manufactured from bovine blood collected in USDA-inspected abattoirs located in the United States. Serum has not come from cattle born, raised, shipped through, or slaughtered in countries where BSE is known to exist.

    For-Profit:   $21.00 Non-Profit:   $21.00

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  • Iscove's Modified Dulbecco's Medium (IMDM) (ATCC® 30-2005)

    ATCC® Number: 30-2005
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Iscove's Modified Dulbecco's Medium (IMDM) contains 4 mM L-glutamine, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate.

    For-Profit:   $22.00 Non-Profit:   $22.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.