Media Sera Reagents

  • Hypoxanthine - Thymidine (H.T.) 500x concentrate (ATCC® 71-X)

    ATCC® Number: 71-X
    Quantity: 5 x 2 ml vials
    each prepares 1 L of supplement

    Storage: Storage temperature of 2°C to 8°C is recommended
    Applications:

    This reagent is used in hybridoma production as a source of hypoxanthine and thymidine after selection in HAT. H.T. is supplied as five 2.0 mL vials.  Each vial of concentrate can produce one liter of 1X H.T. medium.

    Each vial contains sterile, freeze-dried concentrate of:

             Hypoxanthine        13.610 mg

             Thymidine               3.780 mg

             BSA fraction V      20.000 mg


    For-Profit:   $27.00 Non-Profit:   $27.00

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    Product Format: 5 x 2 ml vials
    each prepares 1 L of supplement
    Comments:
    H.T. is used to maintain hybridoma selection in cell culture. It is supplied in 5 vials of 2 ml each; each vial is sufficient for 1 liter of supplement.
    Each vial contains:
    Hypoxanthine 13.61 mg
    Thymidine 3.78 mg
    BSA fraction V 20.00 mg
  • Dulbecco's Modified Eagle's Medium (DMEM) (ATCC® 30-2002)

    ATCC® Number: 30-2002
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Dulbecco's Modified Eagle's Medium (DMEM) modified to contain 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. 

    For-Profit:   $20.00 Non-Profit:   $20.00

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    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • Vitamin Supplement (ATCC® MD-VS)

    ATCC® Number: MD-VS
    Quantity: Package of 5 (10 mL/vial)
    Storage: Store at -20°C. Avoid freeze/thaw cycling. Do not autoclave.
    Applications:

    ATCC® Vitamin Supplement is used as an additive in many ATCC® recommended media, especially for cultivating extremophiles. It speeds media preparation and reduces possible lab errors by combining several trace mineral ingredients into one sterile solution. Product is provided in an individual unit of use (10 mL).

    For-Profit:   $65.00 Non-Profit:   $65.00

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    Product Format: Package of 5 (10 mL/vial)
  • ROCK Inhibitor Y27632 (ATCC® ACS-3030)

    ATCC® Number: ACS-3030
    Quantity: 10 mg
    Storage: -20°C or colder

    For-Profit:   $195.00 Non-Profit:   $195.00

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    Product Format: dried 10 mg
    Comments:
    Purity is > 98% as confirmed by HPLC analysis. Structure and mass are confirmed by NMR and mass spectrometry.
    Reconstituted product can be aliquoted and stored at -20°C for up to six months. Protect from light.
  • Stem Cell Freezing Media (ATCC® ACS-3020)

    ATCC® Number: ACS-3020
    Quantity: 1 x 20 mL
    Storage: 2°C to 8°C

    For-Profit:   $50.00 Non-Profit:   $50.00

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    Product Format: frozen 1 x 20 mL
    Comments:
    Stem Cell Freezing Media is tested for pH, appearance, and sterility. Recovery, morphology and differentiation of hESCs and hiPSCs are confirmed after cryopreservation and thawing.
  • Calf Bovine Serum, Iron Fortified (ATCC® 30-2030)

    ATCC® Number: 30-2030
    Quantity: 500 mL
    Storage: Store calf bovine serum, iron fortified at -20°C or colder. Do not store frozen calf bovine serum, iron fortified at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing calf bovine serum, iron fortified in aliquots. Calf bovine serum iron fortified is supplied in PETG plastic bottles.
    Applications:

    Iron supplemented and triple filtered through 0.1 μm filters. Each lot of calf bovine serum is tested for sterility and for the ability to support the growth of cells using sequential growth curves. Calf bovine serum, iron fortified, is manufactured from bovine blood collected in USDA-inspected abattoirs located in the United States. Serum has not come from cattle born, raised, shipped through, or slaughtered in countries where BSE is known to exist.

    For-Profit:   $53.00 Non-Profit:   $53.00

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  • Pluripotent Stem Cell SFM XF/FF (ATCC® ACS-3002)

    ATCC® Number: ACS-3002
    Storage: Complete medium is stored at -20°C

    For-Profit:   $230.00 Non-Profit:   $230.00

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    Product Format: frozen
    Comments:
    This ready to use, complete medium, is tested for pH, osmolality, endotoxins and sterility. Each lot of Pluripotent Stem Cell SFM XF/FF is tested functionally with human stem cells to ensure that pluripotency is maintained as determined by expression of pluripotent markers.
  • Vascular Cell Basal Medium (ATCC® PCS-100-030)

    ATCC® Number: PCS-100-030
    Quantity: 475 mL
    Storage: 2°C to 8°C, protect from light
    Applications: Vascular Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system.

    This system is designed to support cells derived from normal human large vessels such as human umbilical vein endothelial cells, aortic endothelial cells or aortic smooth muscle cells, as well as microvascular endothelial cells. Vascular Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of cells derived from the vascular system, Vascular Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit.

    For-Profit:   $50.00 Non-Profit:   $50.00

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  • For-Profit:   $50.00 Non-Profit:   $50.00

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  • Renal Epithelial Cell Basal Medium (ATCC® PCS-400-030)

    ATCC® Number: PCS-400-030
    Quantity: 485 mL
    Storage: 2 to 8°C, protect from light
    Applications: Renal Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This system is designed to support epithelial cells (e.g., renal proximal tubule epithelial cells) derived from normal human kidney. Renal Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of renal epithelial cells (e.g., Primary Renal Proximal Tubule Epithelial Cells, Normal, Human, ATCC PCS-400-010; Primary Renal Cortical Epithelial Cells, Normal, Human, ATCC PCS-400-011; Primary Renal Mixed Epithelial Cells, Normal, Human, ATCC PCS-400-012), Renal Cell Basal Medium must be supplemented with the Renal Epithelial Cell Growth Kit (ATCC PCS-400-040).

    Using Renal Epithelial Cell Basal Medium supplemented with the Renal Epithelial Cell Growth Kit, the growth of renal epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates.

    Optional media supplements:

    1. Gentamicin-Amphotericin B Solution  (ATCC PCS-999-025)
    2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    3. Phenol Red (ATCC PCS-999-001)

    For-Profit:   $50.00 Non-Profit:   $50.00

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