Media Sera Reagents

  • Dulbecco's Modified Eagle's Medium (DMEM) without Phenol Red (ATCC® 30-2601)

    ATCC® Number: 30-2601
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Dulbecco's Modified Eagle's Medium (DMEM) is a basal medium widely used in mammalian cell culture. The medium is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and vitamins. This formulation does not contain phenol red or L-glutamine.

    For-Profit:   $21.00 Non-Profit:   $21.00

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    Product Format: 500 mL
    Comments: Supplement with 4 mM L-glutamine prior to use.
  • Pluripotent Stem Cell SFM XF (ATCC® ACS-3001)

    ATCC® Number: ACS-3001
    Quantity: 500 mL
    Storage: -20°C

    For-Profit:   $135.00 Non-Profit:   $135.00

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  • Modified PYNFH Medium [ATCC medium 1034] (ATCC® 327-X)

    ATCC® Number: 327-X
    Storage: 2 to 8°C
    Applications: This medium is the recommended formulation for the axenic cultivation of small, free-living amoebae: Acanthamoeba, Hartmannella, Naegleria; insect trypanosomes: Crithidia, Herpetomonas, Leptomonas, Phytomonas; and Tetrahymena. It is provided freeze-dried, 1 vial prepares 55 mL of medium. The medium is ready to use upon rehydration with sterile glass-distilled water.

    For-Profit:   $65.00 Non-Profit:   $65.00

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    Product Format: freeze-dried
    Comments:
    The medium is ready to use upon rehydration with sterile glass-distilled water.
  • Hypoxanthine - Aminopterin - Thymidine (H.A.T.) 500x concentrate (ATCC® 69-X)

    ATCC® Number: 69-X
    Quantity: 5 x 2 ml vials, each prepares 1 L of supplement
    Storage: Storage temperature of 2°C to 8°C is recommended.
    Applications:

    This reagent is used in hybridoma production to select hybridomas from normal cell populations. HAT is supplied as five 2.0 mL vials. Each vial of concentrate can produce one liter of 1X HAT medium.

    For-Profit:   $30.00 Non-Profit:   $30.00

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    Product Format: freeze-dried 5 x 2 ml vials, each prepares 1 L of supplement
    Comments:
    It is supplied in 5 vials of 2 ml each; each vial is sufficient for 1 liter of supplement.
    Each vial contains:
    Hypoxanthine 13.610 mg
    Aminopterin 0.176 mg
    Thymidine 3.780 mg
    BSA fraction V 20.000 mg
  • Adipocyte Differentiation Toolkit for Adipose-Derived MSCs and Preadipocytes (ATCC® PCS-500-050)

    ATCC® Number: PCS-500-050
    Quantity: 1 kit
    Storage: -20°C (or -70°C for long-term storage)
    Applications:

    The Adipocyte Differentiation Toolkit for Adipose-derived MSCs and Preadipocytes contains medium and reagents designed to induce adipogenesis in actively proliferating Adipose-Derived Mesenchymal Stem Cells and Preadipocytes with high efficiency, and to support maturation of derived adipocytes during lipid accumulation. The Toolkit is composed of the following components:
    1. One vial containing 1 mL of AD Supplement for use in initiation of adipocyte differentiation
    2. One bottle containing 5 mL of ADM Supplement for use in maintaining adipocyte differentiation
    3. One bottle containing 100 mL of Adipocyte Basal Medium for use in both initiation and maintenance of adipocyte differentiation

    *The Adipocyte Differentiation Toolkit for Adipose-derived MSCs and Preadipocytes provides enough medium and reagents for differentiation of ~6.8 x 105 cells when plated at a recommended density of 18,000 viable cells/cm2 in 4 wells of a 6 well tissue culture format. 



    For-Profit:   $159.00 Non-Profit:   $159.00

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  • Erythrosin B Stain Solution (ATCC® 30-2404)

    ATCC® Number: 30-2404
    Quantity: 40 ml
    Storage: Store Erythrosin B stain solution at room temperature (15°C to 30°C).
    Applications:

    Erythrosin B (0.1% in Phosphate Buffered Saline) is a vital dye used to determine cell viability. Viable cells will actively exclude the dye, while nonviable cells (those whose plasma membrane is damaged) will take up the stain and appear red in color.

    For-Profit:   $17.00 Non-Profit:   $17.00

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    Product Format: 40 ml
    Comments:
    Cell culture tested.
  • Airway Epithelial Cell Basal Medium (ATCC® PCS-300-030)

    ATCC® Number: PCS-300-030
    Quantity: 485 mL
    Storage: 4 to 8°C, protect from light
    Applications:

    Airway Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This serum-free system is designed to support airway epithelial cells derived from normal human lung. Airway Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of various types of airway epithelial cells, Airway Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit. When using this complete media system, the growth of airway epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates. 

    1. For airway epithelial cells derived from bronchial/tracheal tissue (e.g., Primary Bronchial/Tracheal Epithelial Cells, Normal, Human, ATCC PCS-300-010), supplement Airway Epithelial Cell Basal Medium with the Bronchial Epithelial Cell Growth Kit (ATCC PCS-300-040).
    2. For small airway epithelial cells (e.g., Primary Small Airway Epithelial Cells, Normal, Human, ATCC PCS-301-010), supplement Airway Epithelial Cell Basal Medium with the Small Airway Epithelial Cell Growth Kit (ATCC PCS-301-040).
    3. Optional media supplements:
      1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
      2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
      3. Phenol Red (ATCC PCS-999-001)

    For-Profit:   $49.00 Non-Profit:   $49.00

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  • For-Profit:   $25.00 Non-Profit:   $25.00

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  • Fibroblast Basal Medium (ATCC® PCS-201-030)

    ATCC® Number: PCS-201-030
    Quantity: 480 ml
    Storage: 2 to 8°C; protect from light
    Applications:

    Fibroblast Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This system is designed to support fibroblasts derived from normal human epidermis. Fibroblast Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts.

    1. To support the proliferation and plating efficiency of fibroblasts (e.g., Dermal Fibroblasts, Normal, Human Neonatal [ATCC PCS-201-010], Dermal Fibroblasts, Normal, Human Neonatal, Mitomycin C Treated [ATCC PCS-201-010]), Fibroblast Basal Medium must be supplemented with one of the following kits:
      1. Fibroblast Growth Kit–Serum-Free (ATCC PCS-201-040)
      2. Fibroblast Growth Kit–Low Serum (ATCC PCS-201-041)
    2. Optional media supplements:
      1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
      2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
      3. Phenol Red (ATCC PCS-999-001)

    For-Profit:   $49.00 Non-Profit:   $49.00

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  • Leibovitz's L-15 Medium (ATCC® 30-2008)

    ATCC® Number: 30-2008
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Leibovitz's L-15 Medium contains 2 mM L-glutamine and no sodium bicarbonate.

    For-Profit:   $23.00 Non-Profit:   $23.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    NOTE: This formulation is designed to be used without additional CO2 in the atmosphere of cell cultures.