Media Sera Reagents

  • Penicillin-Streptomycin Solution (ATCC® 30-2300™)

    ATCC® Number: 30-2300 ™
    Quantity: 100 mL
    Storage: -20°C or colder
    Applications:

    10,000 I.U./mL Penicillin, 10,000 (μg/mL) Streptomycin For reducing the chances of microbial contamination in cell culture. Between 0.5 and 1 mL of Penicillin-Streptomycin solution are added to 100 mL of cell culture media for a final concentration of 50 to 100 I.U./mL penicillin and 50 to 100 (μg/mL) streptomycin.

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    Product Format: frozen 100 mL
    Comments: NOTE: Most cell culture technologists avoid using antibiotics and antimycotics for routine culture work. Antibiotics and antimycotics may mask contamination by susceptible bacteria and fungi while permitting mycoplasma to flourish unnoticed. Antibiotics and antimycotics may interfere with the metabolism of sensitive cells in culture. However, one may elect to introduce antibiotics and antimycotics for short periods to primary cultures or as a safeguard while propagating specific valuable stocks.

  • Calf Bovine Serum (ATCC® 30-2030™)

    ATCC® Number: 30-2030 ™
    Quantity: 500 mL
    Storage: Store calf bovine serum at -20°C or colder. Do not store frozen calf bovine serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing calf bovine serum in aliquots. Calf bovine serum is supplied in PETG plastic bottles.
    Applications: Iron supplemented and triple filtered through 0.1 μm filters. Each lot of calf bovine serum is tested for sterility and for the ability to support the growth of cells using sequential growth curves. Calf bovine serum is manufactured from bovine blood collected in USDA-inspected abattoirs located in the United States. Serum has not come from cattle born, raised, shipped through, or slaughtered in countries where BSE is known to exist.

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    Product Format: frozen 500 mL

  • McCoy's 5A Medium (ATCC® 30-2007™)

    ATCC® Number: 30-2007 ™
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    McCoy's 5A Medium is modified to contain 1.5 mM L-glutamine and 2200 mg/L sodium bicarbonate.

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:

    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 2.2 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.

  • Mycoplasma Growth Supplement, 10x solution (ATCC® 20-2207™)

    ATCC® Number: 20-2207 ™
    Quantity: 100 mL
    Storage: 2 to 8°C
    Applications:

    Mycoplasma Growth Supplement is used in broth and agar media to isolate and grow various mycoplasma species. At ATCC the 10x solution is an ingredient in ATCC Medium 988 (Spiroplasma SP-4 Medium).

    For-Profit:   $90.00 Non-Profit:   $90.00

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    Product Format: 100 mL
    Comments:
    Well-defined supplement for mycoplasma culture media. Add aseptically to prepared medium.

  • (ATCC® 30-2102™)

    ATCC® Number: 30-2102™
    Notice: The ATCC number you are searching for is no longer available. You can e-mail Customer Service with the ATCC number to check the status of the item.

    Notice: Product Unavailable

  • Phenol Red (ATCC® PCS-999-001™)

    ATCC® Number: PCS-999-001 ™

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    Product Format: 1.0 mL

  • Fetal Bovine Serum (ATCC® 30-2021™)

    ATCC® Number: 30-2021 ™
    Quantity: 100 mL
    Storage: Store calf bovine serum at -20°C or colder. Do not store frozen calf bovine serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing calf bovine serum in aliquots. Calf bovine serum is supplied in PETG plastic bottles.
    Applications:

    Triple filtered through 0.1 μm filters. Each lot of fetal bovine serum is tested for sterility and for the ability to support the growth of several different cell lines using both sequential growth curves and plating efficiencies. Fetal bovine serum is manufactured from fetal bovine blood collected in USDA-inspected abattoirs located in the United States.

    For-Profit:   $90.00 Non-Profit:   $90.00

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    Product Format: frozen 100 mL

  • L-Glutamine Solution, 200 mM (ATCC® 30-2214™)

    ATCC® Number: 30-2214 ™
    Quantity: 100 mL
    Storage: Store L-glutamine solution frozen at -20°C or colder. Avoid repeated freeze-thaws by dispensing and storing in aliquots.
    Applications: L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture.

    L-Glutamine is also more labile in cell culture solutions than other amino acids. The rate and extent of L-glutamine degradation are related to storage temperatures, age of the product, and pH.

    All cell culture media are formulated to contain appropriate amounts of L-glutamine to support cell growth well beyond the expiration date of the media. However, if L-glutamine is suspected to be a limiting factor during cell culture, a simple test of 'spiking' the medium with a small amount of L-glutamine will determine whether or not more is required. Simply add a small amount of L-glutamine (~ 2 mM final concentration) to the culture medium. If the cell growth rate increases, L-glutamine is most likely deficient and more should be added.

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    Product Format: frozen 100 mL
    Comments:

    Added as a supplement to cell culture media. L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. L-Glutamine is also more labile in cell culture solutions than other amino acids. The rate and extent of L-glutamine degradation are related to storage temperatures, age of the product, and pH. 

    Note: L-glutamine degradation results in the build-up of ammonia which can have a deleterious effect on some cell lines. Use caution when adding more L-glutamine than called for in the original medium formulation (2 to 4 mM is standard). For most cell lines, ammonia toxicity is more critical for cell viability than L-glutamine limitation.

    For more information, please contact ATCC Technical Service.

  • Hypoxanthine - Aminopterin - Thymidine (H.A.T.) 500x concentrate (ATCC® 69-X™)

    ATCC® Number: 69-X ™
    Quantity: 5 x 2 ml vials, each prepares 1 L of supplement
    Storage: Storage temperature of 2°C to 8°C is recommended.
    Applications:

    This reagent is used in hybridoma production to select hybridomas from normal cell populations. HAT is supplied as five 2.0 mL vials. Each vial of concentrate can produce one liter of 1X HAT medium.

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    Product Format: freeze-dried 5 x 2 ml vials, each prepares 1 L of supplement
    Comments:
    It is supplied in 5 vials of 2 ml each; each vial is sufficient for 1 liter of supplement.
    Each vial contains:
    Hypoxanthine 13.610 mg
    Aminopterin 0.176 mg
    Thymidine 3.780 mg
    BSA fraction V 20.000 mg

  • Dulbecco's Modified Eagle's Medium (DMEM) without Phenol Red (ATCC® 30-2601™)

    ATCC® Number: 30-2601 ™
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Dulbecco's Modified Eagle's Medium (DMEM) is a basal medium widely used in mammalian cell culture. The medium is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and vitamins. This formulation does not contain phenol red or L-glutamine.

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    Product Format: 500 mL
    Comments: Supplement with 4 mM L-glutamine prior to use.