Media Sera Reagents

• Airway Epithelial Cell Basal Medium (ATCC^® PCS-300-030^™)

  ATCC^® Number: PCS-300-030 ^™
  Quantity: 485 mL
  Storage: 4 to 8°C, protect from light
  Applications:

  Airway Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC^® Primary Cell Solutions™ system. This serum-free system is designed to support airway epithelial cells derived from normal human lung. Airway Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of various types of airway epithelial cells, Airway Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit. When using this complete media system, the growth of airway epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates. 

  1. For airway epithelial cells derived from bronchial/tracheal tissue (e.g., Primary Bronchial/Tracheal Epithelial Cells, Normal, Human, ATCC PCS-300-010), supplement Airway Epithelial Cell Basal Medium with the Bronchial Epithelial Cell Growth Kit (ATCC PCS-300-040).
  2. For small airway epithelial cells (e.g., Primary Small Airway Epithelial Cells, Normal, Human, ATCC PCS-301-010), supplement Airway Epithelial Cell Basal Medium with the Small Airway Epithelial Cell Growth Kit (ATCC PCS-301-040).
  3. Optional media supplements:
     1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
     2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
     3. Phenol Red (ATCC PCS-999-001)

  For-Profit:   $49.00 Non-Profit:   $49.00

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  Product Format:

  485 mL

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• Hypoxanthine - Aminopterin - Thymidine (H.A.T.) 500x concentrate (ATCC^® 69-X^™)

  ATCC^® Number: 69-X ^™
  Quantity: 5 x 2 ml vials, each prepares 1 L of supplement
  Storage: Storage temperature of 2°C to 8°C is recommended.
  Applications:

  This reagent is used in hybridoma production to select hybridomas from normal cell populations. HAT is supplied as five 2.0 mL vials. Each vial of concentrate can produce one liter of 1X HAT medium.

  For-Profit:   $30.00 Non-Profit:   $30.00

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  Product Format:	freeze-dried 5 x 2 ml vials, each prepares 1 L of supplement
  Comments:	It is supplied in 5 vials of 2 ml each; each vial is sufficient for 1 liter of supplement. Each vial contains: Hypoxanthine 13.610 mg Aminopterin 0.176 mg Thymidine 3.780 mg BSA fraction V 20.000 mg

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• Feeder-Free, Serum-Free Culture System (ATCC^® ACS-3044-K^™)

  ATCC^® Number: ACS-3044-K ^™
  Quantity: 1 Kit

  For-Profit:   $685.00 Non-Profit:   $685.00

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  Product Format:

  frozen 1 Kit

  Comments:

  This kit supplies CellMatrix™ Basement Membrane Gel and cell culture components to support a serum-free, feeder-free cell culture environment, including Pluripotent Stem Cell SFM XF/FF (ATCC ACS-3002), CellMatrix Basement Membrane Gel (ATCC ACS-3035), Stem Cell Dissociation Reagent (ATCC ACS-3010), ROCK Inhibitor Y27632 (ATCC ACS-3030), Stem Cell Freezing Media (ATCC ACS-3020), DMEM: F12 Medium (ATCC 30-2006)and D-PBS (ATCC 30-2200).

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• Prostate Epithelial Cell Basal Medium (ATCC^® PCS-440-030^™)

  ATCC^® Number: PCS-440-030 ^™
  

  For-Profit:   $49.00 Non-Profit:   $49.00

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  Product Format:

  485 ml

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• Chondrocyte Differentiation Tool (ATCC^® PCS-500-051^™)

  ATCC^® Number: PCS-500-051 ^™
  

  For-Profit:   $149.00 Non-Profit:   $149.00

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  Product Format:

  frozen 100 mL

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• Dulbecco's Modified Eagle's Medium (DMEM) without Phenol Red (ATCC^® 30-2601^™)

  ATCC^® Number: 30-2601 ^™
  Quantity: 500 mL
  Storage: Store medium at 2°C to 8°C in the dark when not in use.
  Applications:

  Dulbecco's Modified Eagle's Medium (DMEM) is a basal medium widely used in mammalian cell culture. The medium is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and vitamins. This formulation does not contain phenol red or L-glutamine.

  For-Profit:   $21.00 Non-Profit:   $21.00

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  Product Format:	500 mL
  Comments:	Supplement with 4 mM L-glutamine prior to use.

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• Endothelial Cell Growth Kit-BBE (ATCC^® PCS-100-040^™)

  ATCC^® Number: PCS-100-040 ^™
  

  For-Profit:   $79.00 Non-Profit:   $79.00

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  Product Format:

  frozen 1 kit

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• (ATCC^® ACS-3020^™)

  ATCC^® Number: ACS-3020 ^™
  

  For-Profit:   $50.00 Non-Profit:   $50.00

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• Vascular Smooth Muscle Cell Growth Kit (ATCC^® PCS-100-042^™)

  ATCC^® Number: PCS-100-042 ^™
  

  For-Profit:   $79.00 Non-Profit:   $79.00

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  Product Format:

  frozen 1 kit

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• L-Glutamine Solution, 200 mM (ATCC^® 30-2214^™)

  ATCC^® Number: 30-2214 ^™
  Quantity: 100 mL
  Storage: Store L-glutamine solution frozen at -20°C or colder. Avoid repeated freeze-thaws by dispensing and storing in aliquots.
  Applications: L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture.
  
  L-Glutamine is also more labile in cell culture solutions than other amino acids. The rate and extent of L-glutamine degradation are related to storage temperatures, age of the product, and pH.
  
  All cell culture media are formulated to contain appropriate amounts of L-glutamine to support cell growth well beyond the expiration date of the media. However, if L-glutamine is suspected to be a limiting factor during cell culture, a simple test of 'spiking' the medium with a small amount of L-glutamine will determine whether or not more is required. Simply add a small amount of L-glutamine (~ 2 mM final concentration) to the culture medium. If the cell growth rate increases, L-glutamine is most likely deficient and more should be added.
  

  For-Profit:   $17.00 Non-Profit:   $17.00

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  Product Format:

  frozen 100 mL

  Comments:

  Added as a supplement to cell culture media. L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. L-Glutamine is also more labile in cell culture solutions than other amino acids. The rate and extent of L-glutamine degradation are related to storage temperatures, age of the product, and pH.  Note: L-glutamine degradation results in the build-up of ammonia which can have a deleterious effect on some cell lines. Use caution when adding more L-glutamine than called for in the original medium formulation (2 to 4 mM is standard). For most cell lines, ammonia toxicity is more critical for cell viability than L-glutamine limitation. For more information, please contact ATCC Technical Service.

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