Media Sera Reagents

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  • F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) (ATCC® 30-2004)

    ATCC® Number: 30-2004
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) contains 2 mM L-glutamine and 1500 mg/L sodium bicarbonate.

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    Formulation: Formulation
    Product Format: 500 mL
    Comments: NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • For-Profit:   $81.00 Non-Profit:   $81.00

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  • Trace Mineral Supplement (ATCC® MD-TMS)

    ATCC® Number: MD-TMS
    Quantity: Package of 5 (10 mL/vial)
    Storage: Store at 4 to 8°C
    Applications:

    ATCC® Trace Mineral Supplement is used as an additive in many ATCC® recommended media, especially for cultivating extremophiles. It speeds media preparation and reduces possible lab errors by combining several trace mineral ingredients into one sterile solution. Product is provided in an individual unit of use (10 mL).

    For-Profit:   $65.00 Non-Profit:   $65.00

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    Product Format: Package of 5 (10 mL/vial)
    Comments: Based on Wolfe's formulation
  • Fibroblast Basal Medium (ATCC® PCS-201-030)

    ATCC® Number: PCS-201-030
    Quantity: 480 ml
    Storage: 2 to 8°C; protect from light
    Applications:

    Fibroblast Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This system is designed to support fibroblasts derived from normal human epidermis. Fibroblast Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts.

    1. To support the proliferation and plating efficiency of fibroblasts (e.g., Dermal Fibroblasts, Normal, Human Neonatal [ATCC PCS-201-010], Dermal Fibroblasts, Normal, Human Neonatal, Mitomycin C Treated [ATCC PCS-201-010]), Fibroblast Basal Medium must be supplemented with one of the following kits:
      1. Fibroblast Growth Kit–Serum-Free (ATCC PCS-201-040)
      2. Fibroblast Growth Kit–Low Serum (ATCC PCS-201-041)
    2. Optional media supplements:
      1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
      2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
      3. Phenol Red (ATCC PCS-999-001)

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  • Vitamin Supplement (ATCC® MD-VS)

    ATCC® Number: MD-VS
    Quantity: Package of 5 (10 mL/vial)
    Storage: Store at -20°C. Avoid freeze/thaw cycling. Do not autoclave.
    Applications:

    ATCC® Vitamin Supplement is used as an additive in many ATCC® recommended media, especially for cultivating extremophiles. It speeds media preparation and reduces possible lab errors by combining several trace mineral ingredients into one sterile solution. Product is provided in an individual unit of use (10 mL).

    For-Profit:   $65.00 Non-Profit:   $65.00

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    Product Format: Package of 5 (10 mL/vial)
  • Adipocyte Differentiation Toolkit for Bone Marrow and Umbilical-Derived MSCs (ATCC® PCS-500-053)

    ATCC® Number: PCS-500-053
    Quantity: 1 kit
    Storage: -20°C (or -70°C for long-term storage)
    Applications: The Adipocyte Differentiation Toolkit for Bone Marrow and Umbilical-Derived MSCs contains medium and reagents designed to induce adipogenesis in actively proliferating Bone Marrow and Umbilical-Derived Mesenchymal Stem Cells with high efficiency, and to support maturation of derived adipocytes during lipid accumulation. The Toolkit is composed of the following components: 

    1. One vial containing 1 mL of of ADM Supplement BMMSC/UCMSC for use in initiation of adipocyte differentiation 
    2. One bottle containing 100 mL of Adipocyte Basal Medium for use in both initiation and maintenance of adipocyte differentiation 

    * The Adipocyte Differentiation Toolkit for Bone Marrow and Umbilical-Derived MSCs provides enough medium and reagents for differentiation of ~6.8 x 105 cells when plated at a recommended density of 18,000 viable cells/cm2 in 4 wells of a 6 well tissue culture format.

    For-Profit:   $159.00 Non-Profit:   $159.00

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  • Adipocyte Differentiation Toolkit for Adipose-Derived MSCs and Preadipocytes (ATCC® PCS-500-050)

    ATCC® Number: PCS-500-050
    Quantity: 1 kit
    Storage: -20°C (or -70°C for long-term storage)
    Applications:

    The Adipocyte Differentiation Toolkit for Adipose-derived MSCs and Preadipocytes contains medium and reagents designed to induce adipogenesis in actively proliferating Adipose-Derived Mesenchymal Stem Cells and Preadipocytes with high efficiency, and to support maturation of derived adipocytes during lipid accumulation. The Toolkit is composed of the following components:
    1. One vial containing 1 mL of AD Supplement for use in initiation of adipocyte differentiation
    2. One bottle containing 5 mL of ADM Supplement for use in maintaining adipocyte differentiation
    3. One bottle containing 100 mL of Adipocyte Basal Medium for use in both initiation and maintenance of adipocyte differentiation

    *The Adipocyte Differentiation Toolkit for Adipose-derived MSCs and Preadipocytes provides enough medium and reagents for differentiation of ~6.8 x 105 cells when plated at a recommended density of 18,000 viable cells/cm2 in 4 wells of a 6 well tissue culture format. 



    For-Profit:   $159.00 Non-Profit:   $159.00

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  • Horse Serum (ATCC® 30-2041)

    ATCC® Number: 30-2041
    Quantity: 100 mL
    Storage: Store horse serum at -20°C or colder. Do not store frozen horse serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing horse serum in aliquots. Horse serum is supplied in PETG plastic bottles.
    Applications: Triple filtered through 0.1 μm filters. Each lot of horse serum is tested for sterility and for the ability to support the growth of cells using sequential growth curves. Horse serum is manufactured from blood collected from select donor horse herds maintained within the United States.

    For-Profit:   $19.00 Non-Profit:   $19.00

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  • LYI Giardia Medium [ATCC Medium 2155] (ATCC® PRA-2155)

    ATCC® Number: PRA-2155
    Storage: 2 to 8°C
    Applications: This medium is the recommended formulation for the axenic cultivation of Giardia and Spironucleus.  It is provided freeze-dried, complete with 10% bovine serum, 1 vial prepares 15 mL of medium.

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    Comments:
    Ready to use upon rehydration with sterile glass-distilled water.