Media Sera Reagents Hybri-Care Medium (ATCC® 46-X™) ATCC® Number: 46-X ™ Quantity: 1 Package (prepares 1L of medium) Storage: Store medium at 2°C to 8°C in the dark when not in use. Applications: Hybri-Care medium is based on the formulation used by D.H. Sachs and collaborators for propagation of hybridomas and other fastidious cell lines. Ingredients are essentially those of a modified DMEM and NCTC 135 plus insulin. A combined buffering system of HEPES and added NaHCO3 enables the medium to be used at all stages of hybridoma production from fusion to cloning, including single-cell subcloning. When used with 10% fetal bovine serum, Hybri-Care repeatedly has been found to support vigorous growth of most hybridomas in the ATCC collection. Ozato, K., et al. J. Immunology 124: 533-540, 1980. For-Profit: $18.00 Non-Profit: $18.00 Add to Cart Preview Product Compare Formulation: Formulation Product Format: 1 Package (prepares 1L of medium) Comments: When used with 10% fetal bovine serum, Hybri-Care media repeatedly has been found to support vigorous growth of most hybridomas in the ATCC collection. Product Sheet Certificateof Analysis MSDS Erythrosin B Stain Solution (ATCC® 30-2404™) ATCC® Number: 30-2404 ™ Quantity: 40 ml Storage: Store Erythrosin B stain solution at room temperature (15°C to 30°C). Applications: Erythrosin B (0.1% in Phosphate Buffered Saline) is a vital dye used to determine cell viability. Viable cells will actively exclude the dye, while nonviable cells (those whose plasma membrane is damaged) will take up the stain and appear red in color. For-Profit: $17.00 Non-Profit: $17.00 Add to Cart Preview Product Compare Product Format: 40 ml Comments: Cell culture tested. Product Sheet Certificateof Analysis MSDS Vitamin Supplement (ATCC® MD-VS™) ATCC® Number: MD-VS ™ Quantity: Package of 5 (10 mL/vial) Storage: Store at -20°C. Avoid freeze/thaw cycling. Do not autoclave. Applications: ATCC® Vitamin Supplement is used as an additive in many ATCC® recommended media, especially for cultivating extremophiles. It speeds media preparation and reduces possible lab errors by combining several trace mineral ingredients into one sterile solution. Product is provided in an individual unit of use (10 mL). For-Profit: $65.00 Non-Profit: $65.00 Add to Cart Preview Product Compare Product Format: Package of 5 (10 mL/vial) Product Sheet Certificateof Analysis MSDS Penicillin-Streptomycin-Amphotericin B Solution (ATCC® PCS-999-002™) ATCC® Number: PCS-999-002 ™ For-Profit: $25.00 Non-Profit: $25.00 Add to Cart Preview Product Compare Product Format: frozen 1.0 mL Product Sheet Certificateof Analysis MSDS Soybean Trypsin Inhibitor (ATCC® 30-2104™) ATCC® Number: 30-2104 ™ Quantity: 20 mL (5 mg/mL) Storage: When stored at -20°C, the product is stable until the expiration date on the label. Applications: The use of Soybean Trypsin Inhibitor (SBTI) provides a serum-free option with which to inactivate trypsin when subculturing adherent cells. For-Profit: $72.00 Non-Profit: $72.00 Add to Cart Preview Product Compare Product Format: frozen 20 mL (5 mg/mL) Product Sheet Certificateof Analysis MSDS Polyethylene Glycol (PEG) 4000 (ATCC® 52-X™) ATCC® Number: 52-X ™ Quantity: 5 x 2 gram vials Storage: At the ATCC, PEG-4000 is stored at 2°C to 8°C, but the reagent may be stored at room temperature. Applications: PEG-4000 is used to assist in the fusion of myeloma and spleen cells to form hybridomas. For-Profit: $26.00 Non-Profit: $26.00 Add to Cart Preview Product Compare Product Format: freeze-dried 5 x 2 gram vials Product Sheet Certificateof Analysis MSDS Renal Epithelial Cell Growth Kit (ATCC® PCS-400-040™) ATCC® Number: PCS-400-040 ™ For-Profit: $79.00 Non-Profit: $79.00 Add to Cart Preview Product Compare Product Format: frozen 1 kit Product Sheet Certificateof Analysis MSDS TYGM-9 Medium [ATCC Medium 1171] (ATCC® IV-1171™) ATCC® Number: IV-1171 ™ Storage: 2 to 8°C Applications: This medium is the recommended formulation for the xenic (i.e., bacterized) cultivation of Entamoeba, Dientamoeba, and Endolimax. It is provided in liquid form (8 mL), complete with 3% bovine serum; ready to use. For-Profit: $65.00 Non-Profit: $65.00 Add to Cart Preview Product Compare Product Format: test tube Comments: It is provided in liquid form complete with 3% bovine serum. Product Sheet Certificateof Analysis MSDS DMEM: F-12 Medium (ATCC® 30-2006™) ATCC® Number: 30-2006 ™ Quantity: 500 mL Storage: Store medium at 2°C to 8°C in the dark when not in use. Applications: DMEM: F-12 Medium contains 2.5 mM L-glutamine, 15 mM HEPES, 0.5 mM sodium pyruvate, and 1200 mg/L sodium bicarbonate. For-Profit: $22.00 Non-Profit: $22.00 Add to Cart Preview Product Compare Formulation: Formulation Product Format: 500 mL Comments: NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.2 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2. Product Sheet Certificateof Analysis MSDS Mouse ES Cell Basal Medium (ATCC® SCRR-2011™) ATCC® Number: SCRR-2011 ™ Quantity: 500 mL Storage: Store medium at 2°C to 8°C in the dark when not in use. Applications: General culture conditions to maintain undifferentiated, pluripotent mouse ES cells are now well established, and incremental improvements, such as the development of Mouse ES Cell Basal Medium, are key to the ongoing success of stem cell research. Mouse ES Cell Basal Medium is a sterile liquid tissue culture medium containing phenol red that has been optimized to support the undifferentiated self-renewal cycle related to mouse embryonic stem cell growth. The medium contains a stable glutamine dipeptide, providing a long shelf-life when handled and stored properly medium may be supplemented by addition of ES-Cell Qualified Fetal Bovine Serum (FBS) or a serum replacement designed for mouse ES cells. This medium can support the growth of mouse ES cells on either a mitotically inactivated layer of mouse embryonic fibroblast (MEF) feeder cells, or in a For-Profit: $25.00 Non-Profit: $25.00 Add to Cart Preview Product Compare Product Format: 500 mL Product Sheet Certificateof Analysis MSDS < 1 2 3 4 5 6 7 8 9 10 >