Media Sera Reagents

  • Calf Bovine Serum, Iron Fortified (ATCC® 30-2030)

    ATCC® Number: 30-2030
    Quantity: 500 mL
    Storage: Store calf bovine serum, iron fortified at -20°C or colder. Do not store frozen calf bovine serum, iron fortified at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing calf bovine serum, iron fortified in aliquots. Calf bovine serum iron fortified is supplied in PETG plastic bottles.
    Applications:

    Iron supplemented and triple filtered through 0.1 μm filters. Each lot of calf bovine serum is tested for sterility and for the ability to support the growth of cells using sequential growth curves. Calf bovine serum, iron fortified, is manufactured from bovine blood collected in USDA-inspected abattoirs located in the United States. Serum has not come from cattle born, raised, shipped through, or slaughtered in countries where BSE is known to exist.

    For-Profit:   $52.00 Non-Profit:   $52.00

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  • Stem Cell Dissociation Reagent (ATCC® ACS-3010)

    ATCC® Number: ACS-3010
    Quantity: 250 mg
    Storage: 2°C to 8°C

    For-Profit:   $90.00 Non-Profit:   $90.00

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    Product Format: 250 mg
    Comments:

    Stem Cell Dissociation Reagent is a neutral protease isolated from Bacillus polymyxa that promotes safe and efficient detachment of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) during subcultivation in cell culture. The gentle proteolytic action of the reagent does not damage the cell membrane and supports the growth of hiPSCs in an undifferentiated state. Serum does not inhibit the activity of the reagent. Stem Cell Dissociation Reagent is suitable for hESCs and hiPSCs cultured in feeder-free and feeder-dependent conditions.

  • Dermal Cell Basal Medium (ATCC® PCS-200-030)

    ATCC® Number: PCS-200-030
    Quantity: 485 mL
    Storage: 2 to 8°C, protect from light
    Applications: Dermal Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC Primary Cell Solutions™ system.

    This system is designed to support cells (e.g., keratinocytes, melanocytes) derived from normal human epidermis. Dermal Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. 

    To support the proliferation and plating efficiency of keratinocytes (e.g., Primary Epidermal Keratinocytes, Normal, Human Neonatal Foreskin, ATCC PCS-200-010), Dermal Cell Basal Medium must be supplemented with the Keratinocyte Growth Kit (ATCC PCS-200-040). The complete serum-free (although not animal-free) media is formulated to inhibit fibroblast growth, and the low calcium concentration (0.06 mM) slows differentiation.

    To support the proliferation and plating efficiency of melanocytes (e.g., Primary Epidermal Melanocytes, Normal, Human Neonatal, ATCC PCS-200-012 or Primary Epidermal Melanocytes, Normal, Human Adult, ATCC PCS-200-013), Dermal Cell Basal Medium must be supplemented with either the Melanocyte Growth Kit (ATCC PCS-200-041) or the Adult Melanocyte Growth Kit (ATCC PCS-200-042).

    Using Dermal Cell Basal Medium supplemented with the appropriate growth kit, the growth of keratinocytes and melanocytes are supported without the use of feeder layers, extracellular matrix proteins or other substrates.

    Optional media supplements:

    1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
    2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    3. Phenol Red (ATCC PCS-999-001)

     

    For-Profit:   $49.00 Non-Profit:   $49.00

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  • Polyethylene Glycol (PEG) 1450 (ATCC® 50-X)

    ATCC® Number: 50-X
    Quantity: 5 x 2 gm vials
    Storage: At the ATCC, PEG-1450 is stored at 2°C to 8°C, but the reagent may be stored at room temperature.
    Applications:

    This reagent is used to assist fusion of myeloma and spleen cells to form hybridomas. 

    PEG-1450 is packaged as 2 gram aliquots in amber vials that protect the product from light. PEG is undiluted and is a white solid. It is supplied sterile and pretested for cytotoxicity.


    Molecular Weight 1300-1600

    For-Profit:   $22.00 Non-Profit:   $22.00

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    Product Format: freeze-dried 5 x 2 gm vials
  • Non-Enzymatic Cell Dissociation Solution (ATCC® 30-2103)

    ATCC® Number: 30-2103
    Quantity: 100 mL
    Storage: When stored at 2°C to 8°C, the product is stable until the expiration date on the label.
    Applications:

    Trypsin is the most commonly used enzyme for harvesting cells in culture. A non-enzymatic approach is needed when non-protein and animal-component free materials are required. ATCC Non-Enzymatic Cell Dissociation Solution is a sterile, phenol-red free solution composed of a proprietary mixture of chelators representing an optimized alternative to protein-digesting enzymes. 

    For-Profit:   $26.00 Non-Profit:   $26.00

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    Product Format: 100 mL
    Comments: This product is totally free of animal-derived components.
  • Fetal Bovine Serum (ATCC® 30-2021)

    ATCC® Number: 30-2021
    Quantity: 100 mL
    Storage: Store fetal bovine serum at -20°C or colder. Do not store frozen fetal bovine serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing fetal bovine serum in aliquots. Fetal bovine serum is supplied in PETG plastic bottles.
    Applications:

    Triple filtered through 0.1 μm filters. Each lot of fetal bovine serum is tested for sterility and for the ability to support the growth of several different cell lines using both sequential growth curves and plating efficiencies. Fetal bovine serum is manufactured from fetal bovine blood collected in USDA-inspected abattoirs located in the United States.

    For-Profit:   $90.00 Non-Profit:   $90.00

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  • Polyethylene Glycol (PEG) 4000 (ATCC® 52-X)

    ATCC® Number: 52-X
    Quantity: 5 x 2 gram vials
    Storage: At the ATCC, PEG-4000 is stored at 2°C to 8°C, but the reagent may be stored at room temperature.
    Applications:

    PEG-4000 is used to assist in the fusion of myeloma and spleen cells to form hybridomas.

    For-Profit:   $26.00 Non-Profit:   $26.00

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    Product Format: freeze-dried 5 x 2 gram vials