Media Sera Reagents

  • Non-Enzymatic Cell Dissociation Solution (ATCC® 30-2103)

    ATCC® Number: 30-2103
    Quantity: 100 mL
    Storage: When stored at 2°C to 8°C, the product is stable until the expiration date on the label.
    Applications:

    Trypsin is the most commonly used enzyme for harvesting cells in culture. A non-enzymatic approach is needed when non-protein and animal-component free materials are required. ATCC Non-Enzymatic Cell Dissociation Solution is a sterile, phenol-red free solution composed of a proprietary mixture of chelators representing an optimized alternative to protein-digesting enzymes. 

    For-Profit:   $26.00 Non-Profit:   $26.00

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    Product Format: 100 mL
    Comments: This product is totally free of animal-derived components.
  • Hybri-Care Medium (ATCC® 46-X)

    ATCC® Number: 46-X
    Quantity: 1 Package (prepares 1L of medium)
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Hybri-Care medium is based on the formulation used by D.H. Sachs and collaborators for propagation of hybridomas and other fastidious cell lines.
    A combined buffering system of HEPES and added NaHCO3 enables the medium to be used at all stages of hybridoma production from fusion to cloning, including single-cell subcloning.

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    Formulation: Formulation
    Product Format: 1 Package (prepares 1L of medium)
    Comments:
    Hybri-Care medium is based on the formulation used by D.H. Sachs and collaborators for propagation of hybridomas and other fastidious cell lines. Ingredients are essentially those of a modified DMEM and NCTC 135 plus insulin. A combined buffering system of HEPES and added NaHCO3 enables the medium to be used at all stages of hybridoma production from fusion to cloning, including single-cell subcloning.

    When used with 10% fetal bovine serum, Hybri-Care repeatedly has been found to support vigorous growth of most hybridomas in the ATCC collection.

    Ozato, K., et al. J. Immunology 124: 533-540, 1980.
  • RPMI-1640 Medium (ATCC® 30-2001)

    ATCC® Number: 30-2001
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: RPMI-1640 medium modified to contain 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate, for use in incubators using 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • AlphaMEM with Nucleosides without L-Glutamine. (ATCC® 30-2604)

    ATCC® Number: 30-2604
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    AlphaMEM (Alpha Minimum Essential Medium) is an enriched modification of MEM that is widely used in mammalian cell culture. This formulation is made in Earle’s salts and contains ribonucleosides and deoxyribonucleosides.

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    Product Format: 500 mL
    Comments: Supplement with 2 mM L-glutamine prior to use.
  • Hypoxanthine - Thymidine (H.T.) 500x concentrate (ATCC® 71-X)

    ATCC® Number: 71-X
    Quantity: 5 x 2 ml vials
    each prepares 1 L of supplement

    Storage: Storage temperature of 2°C to 8°C is recommended
    Applications:

    This reagent is used in hybridoma production as a source of hypoxanthine and thymidine after selection in HAT. H.T. is supplied as five 2.0 mL vials.  Each vial of concentrate can produce one liter of 1X H.T. medium.

    Each vial contains sterile, freeze-dried concentrate of:

             Hypoxanthine        13.610 mg

             Thymidine               3.780 mg

             BSA fraction V      20.000 mg


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    Product Format: 5 x 2 ml vials
    each prepares 1 L of supplement
    Comments:
    H.T. is used to maintain hybridoma selection in cell culture. It is supplied in 5 vials of 2 ml each; each vial is sufficient for 1 liter of supplement.
    Each vial contains:
    Hypoxanthine 13.61 mg
    Thymidine 3.78 mg
    BSA fraction V 20.00 mg
  • DMEM: F-12 Medium (ATCC® 30-2006)

    ATCC® Number: 30-2006
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    DMEM: F-12 Medium contains 2.5 mM L-glutamine, 15 mM HEPES, 0.5 mM sodium pyruvate, and 1200 mg/L sodium bicarbonate.

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    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.2 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • Renal Epithelial Cell Basal Medium (ATCC® PCS-400-030)

    ATCC® Number: PCS-400-030
    Quantity: 485 mL
    Storage: 2 to 8°C, protect from light
    Applications: Renal Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This system is designed to support epithelial cells (e.g., renal proximal tubule epithelial cells) derived from normal human kidney. Renal Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of renal epithelial cells (e.g., Primary Renal Proximal Tubule Epithelial Cells, Normal, Human, ATCC PCS-400-010; Primary Renal Cortical Epithelial Cells, Normal, Human, ATCC PCS-400-011; Primary Renal Mixed Epithelial Cells, Normal, Human, ATCC PCS-400-012), Renal Cell Basal Medium must be supplemented with the Renal Epithelial Cell Growth Kit (ATCC PCS-400-040).

    Using Renal Epithelial Cell Basal Medium supplemented with the Renal Epithelial Cell Growth Kit, the growth of renal epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates.

    Optional media supplements:

    1. Gentamicin-Amphotericin B Solution  (ATCC PCS-999-025)
    2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    3. Phenol Red (ATCC PCS-999-001)

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