Media Sera Reagents

  • Horse Serum (ATCC® 30-2041)

    ATCC® Number: 30-2041
    Quantity: 100 mL
    Storage: Store horse serum at -20°C or colder. Do not store frozen horse serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing horse serum in aliquots. Horse serum is supplied in PETG plastic bottles.
    Applications: Triple filtered through 0.1 μm filters. Each lot of horse serum is tested for sterility and for the ability to support the growth of cells using sequential growth curves. Horse serum is manufactured from blood collected from select donor horse herds maintained within the United States.

    For-Profit:   $18.00 Non-Profit:   $18.00

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  • Calf Bovine Serum, Iron Fortified (ATCC® 30-2030)

    ATCC® Number: 30-2030
    Quantity: 500 mL
    Storage: Store calf bovine serum, iron fortified at -20°C or colder. Do not store frozen calf bovine serum, iron fortified at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing calf bovine serum, iron fortified in aliquots. Calf bovine serum iron fortified is supplied in PETG plastic bottles.
    Applications:

    Iron supplemented and triple filtered through 0.1 μm filters. Each lot of calf bovine serum is tested for sterility and for the ability to support the growth of cells using sequential growth curves. Calf bovine serum, iron fortified, is manufactured from bovine blood collected in USDA-inspected abattoirs located in the United States. Serum has not come from cattle born, raised, shipped through, or slaughtered in countries where BSE is known to exist.

    For-Profit:   $52.00 Non-Profit:   $52.00

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  • Mouse ES Cell Basal Medium (ATCC® SCRR-2011)

    ATCC® Number: SCRR-2011
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: General culture conditions to maintain undifferentiated,  pluripotent mouse ES cells are now well established, and  incremental improvements, such as the development of  Mouse ES Cell Basal Medium, are key to the ongoing  success of stem cell research.

    Mouse ES Cell Basal Medium is a sterile liquid tissue culture  medium containing phenol red that has been optimized to  support the undifferentiated self-renewal cycle related to  mouse embryonic stem cell growth.

    The medium contains a stable glutamine dipeptide, providing  a long shelf-life when handled and stored properly medium may be supplemented by addition of ES-Cell  Qualified Fetal Bovine Serum (FBS) or a serum replacement  designed for mouse ES cells. This medium can support the growth of mouse ES cells on  either a mitotically inactivated layer of mouse embryonic  fibroblast (MEF) feeder cells, or in a feeder-free environment using gelatin-coated plates.

    For-Profit:   $25.00 Non-Profit:   $25.00

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  • Fetal Bovine Serum (ATCC® 30-2020)

    ATCC® Number: 30-2020
    Quantity: 500 mL
    Storage: Store fetal bovine serum at -20°C or colder. Do not store frozen fetal bovine serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing fetal bovine serum in aliquots. Fetal bovine serum is supplied in PETG plastic bottles.
    Applications: Triple filtered through 0.1 μm filters. Each lot of fetal bovine serum is tested for sterility and for the ability to support the growth of several different cell lines using both sequential growth curves and plating efficiencies. Fetal bovine serum is manufactured from fetal bovine blood collected in USDA-inspected abattoirs located in the United States.

    For-Profit:   $309.00 Non-Profit:   $309.00

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  • MCDB 131 Medium without L-Glutamine (ATCC® 30-2603)

    ATCC® Number: 30-2603
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    MCDB 131 Medium provides a defined and nutritionally balanced environment for mammalian cell culture. The medium includes trace elements and higher levels of certain vitamins and amino acids compared to basal medium. MCDB 131 Medium was originally developed for human microvascular endothelial cell (HUVEC) culture, but supports the growth of other cell types, including hepatocytes, smooth muscle cells, and cardiac myocytes.

    For-Profit:   $36.00 Non-Profit:   $36.00

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    Product Format: 500 mL
    Comments: Supplement with glutamine prior to use. For vascular endothelial cells, 10 mM glutamine (final concentration) is recommended. For other cell lines, 2 mM glutamine (final concentration) is generally required; however, the optimal concentration is dependent on your cell line of interest.
  • Polyethylene Glycol (PEG) 4000 (ATCC® 52-X)

    ATCC® Number: 52-X
    Quantity: 5 x 2 gram vials
    Storage: At the ATCC, PEG-4000 is stored at 2°C to 8°C, but the reagent may be stored at room temperature.
    Applications:

    PEG-4000 is used to assist in the fusion of myeloma and spleen cells to form hybridomas.

    For-Profit:   $26.00 Non-Profit:   $26.00

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    Product Format: freeze-dried 5 x 2 gram vials
  • Hypoxanthine - Thymidine (H.T.) 500x concentrate (ATCC® 71-X)

    ATCC® Number: 71-X
    Quantity: 5 x 2 ml vials
    each prepares 1 L of supplement

    Storage: Storage temperature of 2°C to 8°C is recommended
    Applications:

    This reagent is used in hybridoma production as a source of hypoxanthine and thymidine after selection in HAT. H.T. is supplied as five 2.0 mL vials.  Each vial of concentrate can produce one liter of 1X H.T. medium.

    Each vial contains sterile, freeze-dried concentrate of:

             Hypoxanthine        13.610 mg

             Thymidine               3.780 mg

             BSA fraction V      20.000 mg


    For-Profit:   $26.00 Non-Profit:   $26.00

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    Product Format: 5 x 2 ml vials
    each prepares 1 L of supplement
    Comments:
    H.T. is used to maintain hybridoma selection in cell culture. It is supplied in 5 vials of 2 ml each; each vial is sufficient for 1 liter of supplement.
    Each vial contains:
    Hypoxanthine 13.61 mg
    Thymidine 3.78 mg
    BSA fraction V 20.00 mg
  • McCoy's 5A Medium (ATCC® 30-2007)

    ATCC® Number: 30-2007
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    McCoy's 5A Medium is modified to contain 1.5 mM L-glutamine and 2200 mg/L sodium bicarbonate.

    For-Profit:   $18.00 Non-Profit:   $18.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:

    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 2.2 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.