Media Sera Reagents

  • Airway Epithelial Cell Basal Medium (ATCC® PCS-300-030)

    ATCC® Number: PCS-300-030
    Quantity: 485 mL
    Storage: 4 to 8°C, protect from light
    Applications:

    Airway Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This serum-free system is designed to support airway epithelial cells derived from normal human lung. Airway Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of various types of airway epithelial cells, Airway Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit. When using this complete media system, the growth of airway epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates. 

    1. For airway epithelial cells derived from bronchial/tracheal tissue (e.g., Primary Bronchial/Tracheal Epithelial Cells, Normal, Human, ATCC PCS-300-010), supplement Airway Epithelial Cell Basal Medium with the Bronchial Epithelial Cell Growth Kit (ATCC PCS-300-040).
    2. For small airway epithelial cells (e.g., Primary Small Airway Epithelial Cells, Normal, Human, ATCC PCS-301-010), supplement Airway Epithelial Cell Basal Medium with the Small Airway Epithelial Cell Growth Kit (ATCC PCS-301-040).
    3. Optional media supplements:
      1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
      2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
      3. Phenol Red (ATCC PCS-999-001)

    For-Profit:   $50.00 Non-Profit:   $50.00

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  • Non-Enzymatic Cell Dissociation Solution (ATCC® 30-2103)

    ATCC® Number: 30-2103
    Quantity: 100 mL
    Storage: When stored at 2°C to 8°C, the product is stable until the expiration date on the label.
    Applications:

    Trypsin is the most commonly used enzyme for harvesting cells in culture. A non-enzymatic approach is needed when non-protein and animal-component free materials are required. ATCC Non-Enzymatic Cell Dissociation Solution is a sterile, phenol-red free solution composed of a proprietary mixture of chelators representing an optimized alternative to protein-digesting enzymes. 

    For-Profit:   $27.00 Non-Profit:   $27.00

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    Product Format: 100 mL
    Comments: This product is totally free of animal-derived components.
  • Dimethylsulfoxide (DMSO) (ATCC® 4-X)

    ATCC® Number: 4-X
    Quantity: 5 x 5 mL vials
    Storage: Store DMSO at 2°C to 8°C when not in use. It may be solid at this temperature. However, it may also be stored at room temperature where it remains a liquid.
    Applications:

    DMSO is used as a cyroprotectant for the cryopreservation of cell lines. ATCC DMSO is cell culture grade and has been tested to assure nontoxicity and sterility.

    For-Profit:   $47.00 Non-Profit:   $47.00

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  • Polyethylene Glycol (PEG) 4000 (ATCC® 52-X)

    ATCC® Number: 52-X
    Quantity: 5 x 2 gram vials
    Storage: At the ATCC, PEG-4000 is stored at 2°C to 8°C, but the reagent may be stored at room temperature.
    Applications:

    PEG-4000 is used to assist in the fusion of myeloma and spleen cells to form hybridomas.

    For-Profit:   $27.00 Non-Profit:   $27.00

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    Product Format: freeze-dried 5 x 2 gram vials
  • Calf Bovine Serum, Iron Fortified (ATCC® 30-2030)

    ATCC® Number: 30-2030
    Quantity: 500 mL
    Storage: Store calf bovine serum, iron fortified at -20°C or colder. Do not store frozen calf bovine serum, iron fortified at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing calf bovine serum, iron fortified in aliquots. Calf bovine serum iron fortified is supplied in PETG plastic bottles.
    Applications:

    Iron supplemented and triple filtered through 0.1 μm filters. Each lot of calf bovine serum is tested for sterility and for the ability to support the growth of cells using sequential growth curves. Calf bovine serum, iron fortified, is manufactured from bovine blood collected in USDA-inspected abattoirs located in the United States. Serum has not come from cattle born, raised, shipped through, or slaughtered in countries where BSE is known to exist.

    For-Profit:   $53.00 Non-Profit:   $53.00

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  • Water, Cell Culture Grade (ATCC® 30-2205)

    ATCC® Number: 30-2205
    Quantity: 500 mL
    Storage: Store cell culture grade water at room temperature (15°C to 30°C). Shelf life of 36 months when properly stored.
    Applications:

    For use as a diluent or for reconstituting powdered media and salt solutions.

    For-Profit:   $11.00 Non-Profit:   $11.00

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    Product Format: 500 mL
    Comments:
    Cell culture tested.
  • L-Alanyl-L-Glutamine, 200 mM Solution in 0.85% NaCl. (ATCC® 30-2115)

    ATCC® Number: 30-2115
    Quantity: 100 mL
    Storage: Store L-alanyl-L-glutamine solution at 2°C to 30°C.
    Applications:

    L-glutamine (200 mM solution in 0.85% NaCl) is an essential amino acid required by virtually all mammalian and insect cells grown in culture. L‑alanyl-L-glutamine can be used as a direct substitute for L-glutamine at equimolar concentrations (2 to 4 mM for most applications). Whereas L-glutamine is labile in cell culture medium and its degradation results in a deleterious build-up of ammonia, the L-alanyl-L-glutamine dipeptide is very stable with minimal ammonia production. Once inside the cell, the dipeptide is split by aminopeptidases into L-alanine and L-glutamine.

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  • LYI Entamoeba Medium (ATCC® PRA-2154)

    ATCC® Number: PRA-2154
    Storage: 2 to 8°C or colder
    Applications: This medium is the recommended formulation for the axenic cultivation of Entamoeba, Hypotrichomonas, Mastigamoeba, Pentatrichomonas, Tetratrichomonas, Trichomitus, Trichomonas, and Tritrichomonas. It is provided freeze-dried, complete with 10% (1.5 mL) bovine serum, 1 vial prepares 15 mL of medium.

    For-Profit:   $65.00 Non-Profit:   $65.00

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    Product Format: freeze-dried
    Comments:
    Ready to use upon rehydration with sterile glass-distilled water.

    Media may not support growth of some strains; efficacy for growth of a particular strain should be determined empirically prior to regular work.