Media Sera Reagents

  • Serum-Free Cell Freezing Medium (ATCC® 30-2600)

    ATCC® Number: 30-2600
    Quantity: 20 mL
    Storage: 2°C to 8°C
    Applications: ATCC® Serum-Free Cell Freezing Medium is a sterile, ready-to-use medium suitable for the cryopreservation of adherent and suspension cell cultures.

    Cells cryopreserved using Serum-Free Freezing Medium show levels of viability and percent attachment (adherent cells) that are comparable to cells preserved in DMSO and FBS. Serum-Free Cell Freezing Medium can be used for both cells cultured in serum-supplemented growth medium as well as cells grown under serum-free conditions.

    For-Profit:   $17.00 Non-Profit:   $17.00

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    Product Format: 20 mL
    Comments:

    Animal Component-Free, Protein-Free Cell Freezing Medium

    Cells cryopreserved using Serum-Free Freezing Medium show levels of viability and percent attachment (adherent cells) that are comparable to cells preserved in DMSO and FBS. Serum-Free Cell Freezing Medium can be used for both cells cultured in serum-supplemented growth medium as well as cells grown under serum-free conditions.

  • Dermal Cell Basal Medium (ATCC® PCS-200-030)

    ATCC® Number: PCS-200-030
    Quantity: 485 mL
    Storage: 2 to 8°C, protect from light
    Applications: Dermal Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC Primary Cell Solutions™ system.

    This system is designed to support cells (e.g., keratinocytes, melanocytes) derived from normal human epidermis. Dermal Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. 

    To support the proliferation and plating efficiency of keratinocytes (e.g., Primary Epidermal Keratinocytes, Normal, Human Neonatal Foreskin, ATCC PCS-200-010), Dermal Cell Basal Medium must be supplemented with the Keratinocyte Growth Kit (ATCC PCS-200-040). The complete serum-free (although not animal-free) media is formulated to inhibit fibroblast growth, and the low calcium concentration (0.06 mM) slows differentiation.

    To support the proliferation and plating efficiency of melanocytes (e.g., Primary Epidermal Melanocytes, Normal, Human Neonatal, ATCC PCS-200-012 or Primary Epidermal Melanocytes, Normal, Human Adult, ATCC PCS-200-013), Dermal Cell Basal Medium must be supplemented with either the Melanocyte Growth Kit (ATCC PCS-200-041) or the Adult Melanocyte Growth Kit (ATCC PCS-200-042).

    Using Dermal Cell Basal Medium supplemented with the appropriate growth kit, the growth of keratinocytes and melanocytes are supported without the use of feeder layers, extracellular matrix proteins or other substrates.

    Optional media supplements:

    1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
    2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    3. Phenol Red (ATCC PCS-999-001)

     

    For-Profit:   $49.00 Non-Profit:   $49.00

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  • HEKPlus SFM (ATCC® ACS-4002)

    ATCC® Number: ACS-4002
    Quantity: 1 L
    Storage: Store at 2°C to 8°C. Do not freeze. Protect from light.

    For-Profit:   $48.00 Non-Profit:   $48.00

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    Product Format: 1 L
    Comments:
    HEKPlus SFM should be supplemented with L-alanyl-glutamine or L-glutamine prior to use. The medium contains 0.1% Poloxamer 188, a surfactant shown to enhance cell yield and reduce cell aggregation. HEKPlus SFM also includes human transferrin and recombinant human insulin that have been shown to improve cell proliferation.

    This medium has been optimized for use with ATCC HEK293T/17 SF suspension cells (ATCC ACS-4500) and GeneX Plus transfection reagent (ATCC ACS-4004) to reproducibly express a wide range of proteins at high levels. HEK293T/17 SF cells are pre-adapted to the medium and can be cultured in suspension and directly transfected in HEKPlus SFM using GeneXPlus transfection reagent. Transfection complexes can be prepared in the HEKPlus medium as well. In general, HEK293 cells currently adapted to serum-free medium can be subcultured directly using higher seeding densities. For cells currently adapted to serum containing medium, sequential adaptation to HEKPlus SFM is recommended.

  • Iscove's Modified Dulbecco's Medium (IMDM) (ATCC® 30-2005)

    ATCC® Number: 30-2005
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Iscove's Modified Dulbecco's Medium (IMDM) contains 4 mM L-glutamine, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate.

    For-Profit:   $22.00 Non-Profit:   $22.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • MEM Nonessential Amino Acid Solution, 100x Solution (ATCC® 30-2116)

    ATCC® Number: 30-2116
    Quantity: 100 mL
    Storage: Store MEM Nonessential Amino Acid Solution at 2°C to 8°C
    Applications:

    Added as a supplement to minimal basal media. The nonessential amino acids in this solution are 100 times the concentration found in MEM-alpha Medium.

    For-Profit:   $13.00 Non-Profit:   $13.00

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    Formulation: Formulation
    Product Format: 100 mL
    Comments:
    Added as a supplement to minimal basal media. Contains the nonessential amino acids found in MEM-alpha medium at 100x concentration.
  • For-Profit:   $695.00 Non-Profit:   $695.00

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    Formulation: Formulation
    Product Format: frozen 1 Kit
    Comments:
    This kit supplies ready-to-plate irradiated HFF-1 human foreskin fibroblasts (HFF) (ATCC SCRC 1041.1) as the feeder cell layer and medium components including DMEM: F12 Medium (ATCC 30-2006), ES-Qualified FBS (ATCC SCRR-30-2020), L-Alanyl-L-Glutamine (ATCC 30-2115), MEM Nonessential Amino Acid Solution (ATCC 30-2116) and rh bFGF (ATCC ACS-3025), and Stem Cell Dissociation Reagent (ATCC ACS-3010).
  • DMEM: F-12 Medium (ATCC® 30-2006)

    ATCC® Number: 30-2006
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    DMEM: F-12 Medium contains 2.5 mM L-glutamine, 15 mM HEPES, 0.5 mM sodium pyruvate, and 1200 mg/L sodium bicarbonate.

    For-Profit:   $22.00 Non-Profit:   $22.00

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    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.2 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • Stem Cell Freezing Media (ATCC® ACS-3020)

    ATCC® Number: ACS-3020
    Quantity: 1 x 20 mL
    Storage: 2°C to 8°C

    For-Profit:   $50.00 Non-Profit:   $50.00

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    Product Format: frozen 1 x 20 mL
    Comments:
    Stem Cell Freezing Media is tested for pH, appearance, and sterility. Recovery, morphology and differentiation of hESCs and hiPSCs are confirmed after cryopreservation and thawing.