Media Sera Reagents

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  • TYGM-9 Medium [ATCC Medium 1171] (ATCC® IV-1171)

    ATCC® Number: IV-1171
    Storage: 2 to 8°C
    Applications: This medium is the recommended formulation for the xenic (i.e., bacterized) cultivation of Entamoeba, Dientamoeba, and Endolimax. It is provided in liquid form (8 mL), complete with 3% bovine serum; ready to use.

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    Product Format: test tube
    Comments:
    It is provided in liquid form complete with 3% bovine serum.
  • Vascular Cell Basal Medium (ATCC® PCS-100-030)

    ATCC® Number: PCS-100-030
    Quantity: 475 mL
    Storage: 2°C to 8°C, protect from light
    Applications: Vascular Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system.

    This system is designed to support cells derived from normal human large vessels such as human umbilical vein endothelial cells, aortic endothelial cells or aortic smooth muscle cells, as well as microvascular endothelial cells. Vascular Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of cells derived from the vascular system, Vascular Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit.

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  • Dulbecco's Modified Eagle's Medium (DMEM) (ATCC® 30-2002)

    ATCC® Number: 30-2002
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Dulbecco's Modified Eagle's Medium (DMEM) modified to contain 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. 

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    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
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  • Modified PYNFH Medium [ATCC medium 1034] (ATCC® 327-X)

    ATCC® Number: 327-X
    Storage: 2 to 8°C
    Applications: This medium is the recommended formulation for the axenic cultivation of small, free-living amoebae, including Hartmannella, Naegleria, Willaertia, Vahlkampfia, Capsaspora, Nuclearia; the trypanosomatids Crithidia, Blastocrithidia, Herpetomonas, Leptomonas, Phytomonas; and certain ciliates such as Tetrahymena, Dexiostoma, and Glaucoma. It is provided freeze-dried, 1 vial prepares 55 mL of medium. The medium is ready to use upon rehydration with sterile glass-distilled water.

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    Product Format: freeze-dried
    Comments:
    The medium is ready to use upon rehydration with sterile glass-distilled water.
  • L-Glutamine Solution, 200 mM (ATCC® 30-2214)

    ATCC® Number: 30-2214
    Quantity: 100 mL
    Storage: Store L-glutamine solution frozen at -20°C or colder. Avoid repeated freeze-thaws by dispensing and storing in aliquots.
    Applications: L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture.

    L-Glutamine is also more labile in cell culture solutions than other amino acids. The rate and extent of L-glutamine degradation are related to storage temperatures, age of the product, and pH.

    All cell culture media are formulated to contain appropriate amounts of L-glutamine to support cell growth well beyond the expiration date of the media. However, if L-glutamine is suspected to be a limiting factor during cell culture, a simple test of 'spiking' the medium with a small amount of L-glutamine will determine whether or not more is required. Simply add a small amount of L-glutamine (~ 2 mM final concentration) to the culture medium. If the cell growth rate increases, L-glutamine is most likely deficient and more should be added.

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    Product Format: frozen 100 mL
    Comments:

    Added as a supplement to cell culture media. L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. L-Glutamine is also more labile in cell culture solutions than other amino acids. The rate and extent of L-glutamine degradation are related to storage temperatures, age of the product, and pH. 

    Note: L-glutamine degradation results in the build-up of ammonia which can have a deleterious effect on some cell lines. Use caution when adding more L-glutamine than called for in the original medium formulation (2 to 4 mM is standard). For most cell lines, ammonia toxicity is more critical for cell viability than L-glutamine limitation.

    For more information, please contact ATCC Technical Service.

  • Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs (ATCC® PCS-500-030)

    ATCC® Number: PCS-500-030
    Quantity: 485 mL
    Storage: 4 to 8°C, protect from light
    Applications:

    Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This low serum (2% FBS) system is designed to support mesenchymal stem cells derived from various normal human tissues including lipoaspirates and umbilical cord. Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of various types of adult stem cells, Mesenchymal Stem Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit. When using this complete media system, the growth of undifferentiated mesenchymal stem cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates. 

    1. For mesenchymal stem cells derived from human lipoaspirates (e.g., Adipose-Derived Mesenchymal Stem Cells; Normal, Human, ATCC PCS-500-011) or umbilical cord (Umbilical Cord-Derived Mesenchymal Stem Cells; Normal, Human, ATCC PCS-500-010), supplement Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs with the Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs –Low serum (ATCC PCS-500-040).
    2. For mesenchymal stem cells derived from bone marrow (Bone Marrow-derived Mesenchymal Stem Cells; Normal, Human, ATCC PCS-500-012) supplement Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs with the Mesenchymal  Stem Cell Growth Kit for Bone Marrow-derived MSCs (ATCC PCS-500-041)
    3. Optional media supplements:
      1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
      2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
      3. Phenol Red (ATCC PCS-999-001)

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    Product Format: 485 mL
    Comments:

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  • Trace Mineral Supplement (ATCC® MD-TMS)

    ATCC® Number: MD-TMS
    Quantity: Package of 5 (10 mL/vial)
    Storage: Store at 4 to 8°C
    Applications:

    ATCC® Trace Mineral Supplement is used as an additive in many ATCC® recommended media, especially for cultivating extremophiles. It speeds media preparation and reduces possible lab errors by combining several trace mineral ingredients into one sterile solution. Product is provided in an individual unit of use (10 mL).

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    Product Format: Package of 5 (10 mL/vial)
    Comments: Based on Wolfe's formulation