• remove Product Category: Cell Lines & Hybridomas

Media Sera Reagents

  • Fetal Bovine Serum (ATCC® 30-2021)

    ATCC® Number: 30-2021
    Quantity: 100 mL
    Storage: Store fetal bovine serum at -20°C or colder. Do not store frozen fetal bovine serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing fetal bovine serum in aliquots. Fetal bovine serum is supplied in PETG plastic bottles.
    Applications:

    Triple filtered through 0.1 μm filters. Each lot of fetal bovine serum is tested for sterility and for the ability to support the growth of several different cell lines using both sequential growth curves and plating efficiencies. Fetal bovine serum is manufactured from fetal bovine blood collected in USDA-inspected abattoirs located in the United States.

    For-Profit:   $95.00 Non-Profit:   $95.00

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  • Dulbecco's Modified Eagle's Medium (DMEM) (ATCC® 30-2002)

    ATCC® Number: 30-2002
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Dulbecco's Modified Eagle's Medium (DMEM) modified to contain 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. 

    For-Profit:   $20.00 Non-Profit:   $20.00

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    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • L-Alanyl-L-Glutamine, 200 mM Solution in 0.85% NaCl. (ATCC® 30-2115)

    ATCC® Number: 30-2115
    Quantity: 100 mL
    Storage: Store L-alanyl-L-glutamine solution at 2°C to 30°C.
    Applications:

    L-glutamine (200 mM solution in 0.85% NaCl) is an essential amino acid required by virtually all mammalian and insect cells grown in culture. L‑alanyl-L-glutamine can be used as a direct substitute for L-glutamine at equimolar concentrations (2 to 4 mM for most applications). Whereas L-glutamine is labile in cell culture medium and its degradation results in a deleterious build-up of ammonia, the L-alanyl-L-glutamine dipeptide is very stable with minimal ammonia production. Once inside the cell, the dipeptide is split by aminopeptidases into L-alanine and L-glutamine.

    For-Profit:   $27.00 Non-Profit:   $27.00

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  • Hank's Balanced Salt Solution (HBSS), 1X (ATCC® 30-2213)

    ATCC® Number: 30-2213
    Quantity: 500 mL
    Storage: Store Hank's BSS at ambient temperatures, between 15°C and 30°C
    Applications: For washing cells, for use as a diluent, or for use as an inorganic base for standard media preparation.

    For-Profit:   $14.00 Non-Profit:   $14.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    Contains no calcium, magnesium, or phenol red.
  • Hypoxanthine - Thymidine (H.T.) 500x concentrate (ATCC® 71-X)

    ATCC® Number: 71-X
    Quantity: 5 x 2 ml vials
    each prepares 1 L of supplement

    Storage: Storage temperature of 2°C to 8°C is recommended
    Applications:

    This reagent is used in hybridoma production as a source of hypoxanthine and thymidine after selection in HAT. H.T. is supplied as five 2.0 mL vials.  Each vial of concentrate can produce one liter of 1X H.T. medium.

    Each vial contains sterile, freeze-dried concentrate of:

             Hypoxanthine        13.610 mg

             Thymidine               3.780 mg

             BSA fraction V      20.000 mg


    For-Profit:   $27.00 Non-Profit:   $27.00

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    Product Format: 5 x 2 ml vials
    each prepares 1 L of supplement
    Comments:
    H.T. is used to maintain hybridoma selection in cell culture. It is supplied in 5 vials of 2 ml each; each vial is sufficient for 1 liter of supplement.
    Each vial contains:
    Hypoxanthine 13.61 mg
    Thymidine 3.78 mg
    BSA fraction V 20.00 mg
  • Water, Cell Culture Grade (ATCC® 30-2205)

    ATCC® Number: 30-2205
    Quantity: 500 mL
    Storage: Store cell culture grade water at room temperature (15°C to 30°C). Shelf life of 36 months when properly stored.
    Applications:

    For use as a diluent or for reconstituting powdered media and salt solutions.

    For-Profit:   $11.00 Non-Profit:   $11.00

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    Product Format: 500 mL
    Comments:
    Cell culture tested.
  • Polyethylene Glycol (PEG) 1450 (ATCC® 50-X)

    ATCC® Number: 50-X
    Quantity: 5 x 2 gm vials
    Storage: At the ATCC, PEG-1450 is stored at 2°C to 8°C, but the reagent may be stored at room temperature.
    Applications:

    This reagent is used to assist fusion of myeloma and spleen cells to form hybridomas. 

    PEG-1450 is packaged as 2 gram aliquots in amber vials that protect the product from light. PEG is undiluted and is a white solid. It is supplied sterile and pretested for cytotoxicity.


    Molecular Weight 1300-1600

    For-Profit:   $23.00 Non-Profit:   $23.00

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    Product Format: freeze-dried 5 x 2 gm vials
  • Calf Bovine Serum, Iron Fortified (ATCC® 30-2030)

    ATCC® Number: 30-2030
    Quantity: 500 mL
    Storage: Store calf bovine serum, iron fortified at -20°C or colder. Do not store frozen calf bovine serum, iron fortified at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing calf bovine serum, iron fortified in aliquots. Calf bovine serum iron fortified is supplied in PETG plastic bottles.
    Applications:

    Iron supplemented and triple filtered through 0.1 μm filters. Each lot of calf bovine serum is tested for sterility and for the ability to support the growth of cells using sequential growth curves. Calf bovine serum, iron fortified, is manufactured from bovine blood collected in USDA-inspected abattoirs located in the United States. Serum has not come from cattle born, raised, shipped through, or slaughtered in countries where BSE is known to exist.

    For-Profit:   $53.00 Non-Profit:   $53.00

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  • Leibovitz's L-15 Medium (ATCC® 30-2008)

    ATCC® Number: 30-2008
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Leibovitz's L-15 Medium contains 2 mM L-glutamine and no sodium bicarbonate.

    For-Profit:   $23.00 Non-Profit:   $23.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    NOTE: This formulation is designed to be used without additional CO2 in the atmosphere of cell cultures.
  • Iscove's Modified Dulbecco's Medium (IMDM) (ATCC® 30-2005)

    ATCC® Number: 30-2005
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Iscove's Modified Dulbecco's Medium (IMDM) contains 4 mM L-glutamine, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate.

    For-Profit:   $22.00 Non-Profit:   $22.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.