Media Sera Reagents Page 1

Melanocyte Growth Kit (ATCC^® PCS-200-041^™)

For-Profit: $109.00 Non-Profit: $109.00

Product Format:

frozen 1 Kit

AlphaMEM without Nucleosides without L-Glutamine (ATCC^® 30-2605^™)

ATCC^® Number: 30-2605 ^™
Quantity: 500 mL
Storage: Store medium at 2°C to 8°C in the dark when not in use.
Applications:

AlphaMEM (Alpha Minimum Essential Medium) is an enriched modification of MEM that is widely used in mammalian cell culture. This formulation is made in Earle’s salts and does not contain nucleosides or L-glutamine.

For-Profit: $24.00 Non-Profit: $24.00

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Product Format:	500 mL
Comments:	Supplement with 2 mM L-glutamine prior to use.

Feeder-Dependent, Serum Culture System (HFF) (ATCC^® ACS-3040-K^™)

ATCC^® Number: ACS-3040-K ^™
Quantity: 1 Kit

For-Profit: $695.00 Non-Profit: $695.00

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Formulation:	Formulation
Product Format:	frozen 1 Kit
Comments:	This kit supplies ready-to-plate irradiated HFF-1 human foreskin fibroblasts (HFF) (ATCC SCRC 1041.1) as the feeder cell layer and medium components including DMEM: F12 Medium (ATCC 30-2006), ES-Qualified FBS (ATCC SCRR-30-2020), L-Alanyl-L-Glutamine (ATCC 30-2115), MEM Nonessential Amino Acid Solution (ATCC 30-2116) and rh bFGF (ATCC ACS-3025), and Stem Cell Dissociation Reagent (ATCC ACS-3010).

Hypoxanthine - Thymidine (H.T.) 500x concentrate (ATCC^® 71-X^™)

ATCC^® Number: 71-X ^™
Quantity: 5 x 2 ml vials, each prepares 1 L of supplement
Storage: Storage temperature of 2°C to 8°C is recommended
Applications:

This reagent is used in hybridoma production as a source of hypoxanthine and thymidine after selection in HAT. H.T. is supplied as five 2.0 mL vials. Each vial of concentrate can produce one liter of 1X H.T. medium.

Each vial contains sterile, freeze-dried concentrate of:

         Hypoxanthine        13.610 mg

         Thymidine               3.780 mg

         BSA fraction V      20.000 mg

For-Profit: $26.00 Non-Profit: $26.00

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Product Format:	5 x 2 ml vials, each prepares 1 L of supplement
Comments:	H.T. is used to maintain hybridoma selection in cell culture. It is supplied in 5 vials of 2 ml each; each vial is sufficient for 1 liter of supplement. Each vial contains: Hypoxanthine 13.61 mg Thymidine 3.78 mg BSA fraction V 20.00 mg

Phosphate Buffered Saline Without Calcium Or Magnesium (ATCC^® SCRR-2201^™)

ATCC^® Number: SCRR-2201 ^™
Quantity: 500 mL
Storage: Store at ambient temperatures, between 15°C and 30°C.
Applications:

For washing cells (including embryonic stem cells), for use as a diluent, or for use as an inorganic base for standard media preparation.

For-Profit: $13.00 Non-Profit: $13.00

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Formulation:	Formulation
Product Format:	500 mL

Mitomycin C (ATCC^® SCRR-3020^™)

ATCC^® Number: SCRR-3020 ^™
Quantity: 2 mg
Storage: When stored at 2°C to 8°C, the product is stable until the expiration date on the label.
Applications:

Mitotic inactivation, by treatment of mouse embryonic fibroblasts (MEFs) and other feeder cell cultures with Mitomycin C, prevents division of the fibroblasts while allowing the cells to survive and produce key proteins such as Leukemia Inhibitory Factor (LIF) that support the self renewal cycle of mouse embryonic stem cells.

The use of Mitomycin C requires the implementation of stringent safety measures during use, since the compound is toxic to both cultured cells and to humans.

The fibroblasts must be extensively washed following treatment to prevent toxic effects to embryonic stem (ES) cells, or other cell types that require the use of a feeder cell layer for propagation.

It produces oxygen radicals and is preferentially toxic to hypoxic cells.

For-Profit: $94.00 Non-Profit: $94.00

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Product Format:

2 mg

Comments:

Embryonic stem cells are best supported in vitro by growth on mitotically arrested feeder cells. Mitotic inactivation, by treatment of mouse embryonic fibroblasts (MEFs) and other feeder cell cultures with Mitomycin C, prevents division of the fibroblasts while allowing the cells to survive and produce key proteins such as Leukemia Inhibitory Factor (LIF) that support the self renewal cycle of mouse embryonic stem cells.

The use of Mitomycin C requires the implementation of stringent safety measures during use, since the compound is toxic to both cultured cells and to humans.

Mitomycin C is an antineoplastic antibiotic that inhibits DNA synthesis by cross-linking DNA at 5' —CpG— 3' sequences. It produces oxygen radicals and is preferentially toxic to hypoxic cells. Care must be taken when preparing the Mitomycin C solution. The fibroblasts must be extensively washed following treatment to prevent toxic effects to embryonic stem (ES) cells, or other cell types that require the use of a feeder cell layer for propagation.

Safety Information
Mitomycin C is a highly toxic substance. Do not swallow. It is carcinogenic and a teratogen. In case of accident, or if you feel unwell, seek medical advice immediately. MSDS available upon request.

CAS Number: 50-07-7
Molecular Weight: 334.3
Molecular Formula: C¹⁵H¹⁸N⁴O⁵

Feeder-Dependent, Serum-Free Culture System (MEF) (ATCC^® ACS-3043-K^™)

ATCC^® Number: ACS-3043-K ^™
Quantity: 1 Kit

For-Profit: $510.00 Non-Profit: $510.00

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Product Format:	frozen 1 Kit
Comments:	This kit supplies ready-to-plate irradiated CF-1 mouse embryonicÂ fibroblastsÂ (MEF) as the feeder cell layer and cell culture components including Pluripotent Stem Cell SFM XF (ATCC ACS-3001), Stem Cell Dissociation Reagent (ATCC ACS-3010), ROCK Inhibitor Y27632 (ATCC ACS-3030), Stem Cell Freezing Media (ATCC ACS-3020), DMEM: F12 Medium (ATCC 30-2006) and D-PBS (ATCC 30-2200)

Dimethylsulfoxide (DMSO) (ATCC^® 4-X^™)

ATCC^® Number: 4-X ^™
Quantity: 5 x 5 mL vials
Storage: Store DMSO at 2°C to 8°C when not in use. It may be solid at this temperature. However, it may also be stored at room temperature where it remains a liquid.
Applications:

DMSO is used as a cyroprotectant for the cryopreservation of cell lines. ATCC DMSO is cell culture grade and has been tested to assure nontoxicity and sterility.

For-Profit: $45.00 Non-Profit: $45.00

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Product Format:

5 x 5 mL vials

Fetal Bovine Serum (ATCC^® 30-2021^™)

ATCC^® Number: 30-2021 ^™
Quantity: 100 mL
Storage: Store fetal bovine serum at -20°C or colder. Do not store frozen fetal bovine serum at temperatures above 20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing fetal bovine serum in aliquots. Fetal bovine serum is supplied in PETG plastic bottles.
Applications:

Triple filtered through 0.1 μm filters. Each lot of fetal bovine serum is tested for sterility and for the ability to support the growth of several different cell lines using both sequential growth curves and plating efficiencies. Fetal bovine serum is manufactured from fetal bovine blood collected in USDA-inspected abattoirs located in the United States.

For-Profit: $90.00 Non-Profit: $90.00

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Product Format:

frozen 100 mL

VeroPlus SFM (ATCC^® ACS-4001^™)

ATCC^® Number: ACS-4001 ^™
Quantity: 500 mL
Storage: Store product at 2°C to 8°C. Do not freeze.

For-Profit: $40.00 Non-Profit: $40.00

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Product Format:

500 mL

Comments:

Each lot of VeroPlus SFM is tested functionally for growth characteristics and morphology with Vero cells. Additionally, the media is assayed for pH, osmolality, endotoxins and sterility.

Supplementing with L- Glutamine or L-alanyl-L-Glutamine is required before use.

Media Sera Reagents

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