HO-13-4 (ATCC® TIB-99)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Thy-1.2
Cellular Products
immunoglobulin; monoclonal antibody; against Thy-1.2
Comments
Animals were immunized with thymocytes from C3H mice.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody has only a slight cross-reaction with Thy-1.1.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Isotype IgM
Name of Depositor A Marshak-Rothstein
References

Marshak-Rothstein A, et al. Properties and applications of monoclonal antibodies directed against determinants of the Thy-1 locus. J. Immunol. 122: 2491-2497, 1979. PubMed: 87465

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Marshak-Rothstein A, et al. Properties and applications of monoclonal antibodies directed against determinants of the Thy-1 locus. J. Immunol. 122: 2491-2497, 1979. PubMed: 87465