A-427 (ATCC® HTB-53)

Organism: Homo sapiens, human  /  Tissue: lung  /  Disease: carcinoma

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Organism Homo sapiens, human
Tissue
lung
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease carcinoma
Age 52 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype at passage 60, hypotriploid to hypertriploid with abnormalities including dicentrics, minutes and large subtelocentric marker
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Derivation
The A-427 line was derived by D.J. Giard, as indicated in the description for ATCC HTB-41.
Clinical Data
52 years
Caucasian
male
Tumorigenic Yes
Effects
Yes, in nude mice; forms an undifferentiated tumor suggestive of adenocarcinoma
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Interval: every 6 to 8 days
Subcultivation Ratio: 1:2 to 1:6
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 11,13
D5S818: 12
D7S820: 8,12
THO1: 9
TPOX: 8,11
vWA: 17
Isoenzymes
AK-1, 2
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 1-2
PGM3, 1
Name of Depositor DJ Giard
References

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758