SW 954 [SW-954, SW954] (ATCC® HTB-117)

Organism: Homo sapiens, human  /  Tissue: vulva  /  Disease: grade II, squamous cell carcinoma

Organism Homo sapiens, human
Tissue vulva
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease grade II, squamous cell carcinoma
Age 86 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype pseudodiploid; modal number = 46.
The rate of higher ploidies was 16%. The t(3q;11p), der(11)t(11;?)(q13;?), and M3 were common to all cells. The der(1)t(1;2)(p13;q11) and two others were seen in some cells, and about 10 others were seen only once. All these marker chromosomes were present in one copy per cell., Normal N3, N9, N11 and N18 were single copied, and others were paired. The X was also paired.
Derivation
The SW 954 cell line was initiated by A. Leibovitz in April 1975 at the Scott and White Clinic, Temple, Texas from biopsy tissue of a squamous cell carcinoma of the vulva of an 86 year old female Caucasian.
Clinical Data 86 years
Caucasian
female
Antigen Expression
blood type A; Rh-
Comments

The histopathology of the surgical specimen was determined at the Scott and White Clinic to be a grade II carcinoma.

A frozen ampule of the line at passage 5 was transferred to the ATCC in January, 1982.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: 1:3 to 1:6
Medium Renewal: Every 2 to 3 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions Temperature: 37°C
Atmosphere: Air, 100%. A CO2 and air mixture is detrimental to cells for cultivation.
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 9,11
D16S539: 11
D5S818: 11,12
D7S820: 11,13
THO1: 8,9.3
TPOX: 8
vWA: 14,15
Isoenzymes
AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 1-2
PGM1, 1
PGM3, 1-2
Passage History
A frozen ampule of the line at passage 5 was transferred to the ATCC in January, 1982.
References

Goodrum FD, Ornelles DA. The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle. J. Virol. 71: 548-561, 1997. PubMed: 8985383

Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Basic Documentation
References

Goodrum FD, Ornelles DA. The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle. J. Virol. 71: 548-561, 1997. PubMed: 8985383

Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.