Hybridoma 231 (ATCC® HB-9401)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Lymphoma

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Lymphoma
Applications
The antibody reacts preferentially with canine monocytes and granulocytes, but not with lymphocytes.
It cross-reacts with human granulocytes.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against canine lymphoma cells
Cellular Products
immunoglobulin; monoclonal antibody; against canine lymphoma cells
Comments
Animals were immunized with canine lymphoma cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts preferentially with canine monocytes and granulocytes, but not with lymphocytes.
It cross-reacts with human granulocytes.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
fetal bovine serum, 95%; DMSO, 5%
Isotype IgG2a
Name of Depositor Wistar Institute
References

Steplewski Z, Jeglum KA. Monoclonal antibodies against lymphoma-associated antigens, hybrid cell lines producing these antibodies. US Patent 5,169,775 dated Dec 8 1992

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Steplewski Z, Jeglum KA. Monoclonal antibodies against lymphoma-associated antigens, hybrid cell lines producing these antibodies. US Patent 5,169,775 dated Dec 8 1992