W4F.5B (ATCC® HB-9282)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Genes Expressed
immunoglobulin; monoclonal antibody; against a major framework determinant on human T cell antigen receptor
Cellular Products
immunoglobulin; monoclonal antibody; against a major framework determinant on human T cell antigen receptor
Comments
No other information is available.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2a
Population Doubling Time 24 hrs
Name of Depositor T Cell Sciences, Inc.
References

Brown MC, et al. Assay systems for detecting cell-free T cell antigen receptor related molecules and the clinical utilities of the assays. US Patent 4,845,026 dated Jul 4 1989

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Brown MC, et al. Assay systems for detecting cell-free T cell antigen receptor related molecules and the clinical utilities of the assays. US Patent 4,845,026 dated Jul 4 1989