7G7B6 (ATCC® HB-8784)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Influenza

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Influenza
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human interleukin 2 receptor (interleukin 2, IL-2); CD25
Cellular Products
immunoglobulin; monoclonal antibody; against human interleukin 2 receptor (interleukin 2, IL-2); CD25
Comments
Animals were immunized with influenzavirus stimulated human peripheral blood mononuclear cells.
Spleen cells were fused with Sp2/0 myeloma cells.
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing
    Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Medium Renewal: Every 2 to 3 days
    Cryopreservation
    Freeze medium: Complete growth medium 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Temperature: 37.0°C
    Isotype IgG2a , IgG2a
    Name of Depositor The United States of America
    References

    Biddison W, et al. Soluble interleukin-2 receptor as a disease indicator and a method of assaying the same. US Patent 4,707,443 dated Nov 17 1987

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Biddison W, et al. Soluble interleukin-2 receptor as a disease indicator and a method of assaying the same. US Patent 4,707,443 dated Nov 17 1987