WAPS 12.2 (ATCC® HB-299)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human P-selectin (CD62P)
Cellular Products
immunoglobulin; monoclonal antibody; against human P-selectin (CD62P)
Comments
Animals were immunized with P-selectin transfected mouse L1-2 pre-B cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody blocks leukocyte adhesion to P-selectin and to platelets.
It recognizes P-selectin of a variety of mammals.
Complete Growth Medium RPMI 1640 medium with 4.5 g/L glucose,15 mM HEPES, 2 mM L-glutamine, 0.01 mM nonessential amino acids and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Complete Growth Medium
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain
1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium
pyruvate supplemented with 0.01 mM non-essential amino acids and 0.05 mM
2-mercaptoethanol, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG1
Name of Depositor EC Butcher
References

Jutila MA, et al. Cell surface P- and E-selectin support shear-dependent rolling of bovine gamma/delta T cells. J. Immunol. 153: 3917-3928, 1994. PubMed: 7523509

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Jutila MA, et al. Cell surface P- and E-selectin support shear-dependent rolling of bovine gamma/delta T cells. J. Immunol. 153: 3917-3928, 1994. PubMed: 7523509