CDC 1C42H11 (ATCC® HB-216)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

View Permits

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with and neutralizes CDV of the Rockborn, Onderstepoort and Snyder Hill strains.
The antibody may be useful as a reagent for in vitro testing of CDV vaccines.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against canine distemper virus (CDV)
Cellular Products
immunoglobulin; monoclonal antibody; against canine distemper virus (CDV)
Comments
Animals were immunized with canine distemper virus (Rockborn strain).
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with and neutralizes CDV of the Rockborn, Onderstepoort and Snyder Hill strains.
The antibody may be useful as a reagent for in vitro testing of CDV vaccines.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; bovine calf serum, 2.5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor TO Bunn
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation