B7-24-E1G4 [CMCC 11060] (ATCC® HB-11341)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Biosafety Level 1
Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Comments
Animals were immunized with SF9 insect cells expressing recombinant human B7.
Spleen cells were fused with Sp2/0 myeloma cells.
Complete Growth Medium Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 1% OPI (0.15 mg/ml oxaloacetate, 0.05 mg/ml pyruvate, 0.0082 mg/ml bovine insulin), 90%; heat-inactivated fetal bovine serum, 10%
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2- 3 x 10 exp5 viable cells/ml.
Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
growth medium, 90%; DMSO, 10%
Culture Conditions
Temperature: 37.0°C
Name of Depositor Chiron Corp.
References

de Boer M, Conroy LB. Anti-CD40 monoclonal antibodies capable of blocking B-cell activation. US Patent 5,677,165 dated Oct 14 1997

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

de Boer M, Conroy LB. Anti-CD40 monoclonal antibodies capable of blocking B-cell activation. US Patent 5,677,165 dated Oct 14 1997