L5178Y TK+/- clone (3.7.2C) [TK+/- (clone 3.7.2C)] (ATCC® CRL-9518)

Organism: Mus musculus, mouse  /  Disease: lymphoma

Permits and Restrictions

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Organism Mus musculus, mouse
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphoma
Strain DBA/2
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is a derivative of L5178Y-S (ATCC CRL-1723).
Comments
This line is a derivative of L5178Y-S (ATCC CRL-1723).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.1% Pluronic, 90%; fetal bovine serum, 10%
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 to 2 x 105 viable cells/mL.
Maintain cell density between 2 x 105 and 1 x 106 viable cells/mL.
Medium Renewal: Every 3 to 4 days
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor Mobil Oil Corporation
References

. . Chem. Mutagens 3: pp. 79-103, 1973.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

. . Chem. Mutagens 3: pp. 79-103, 1973.