SC-1 (ATCC® CRL-8756)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte; Epstein-Barr virus (EBV) transforme  / 

Permits and Restrictions

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Organism Homo sapiens, human
Cell Type B lymphocyte; Epstein-Barr virus (EBV) transforme
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS
Comments
The line is homozygous for the sickle cell allele at the hemoglobin beta chain locus.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Medium Renewal: Every 3 to 4 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10 exp5 viable cells/ml.
Maintain cell density between 5 X 10 exp5 and 1.5 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium, 92.5%; DMSO, 7.5%
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,7.3
D13S317: 11,12
D16S539: 10,11
D5S818: 11,12
D7S820: 8,10
THO1: 7
TPOX: 6,9
vWA: 14
Name of Depositor Cetus Corp.
References

Mullis KB, et al. Process for amplifying, detecting, and/or-cloning nucleic acid sequences. US Patent 4,683,195 dated Jul 28 1987

Mullis KB. Process for amplifying nucleic acid sequences. US Patent 4,683,202 dated Jul 28 1987

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Mullis KB, et al. Process for amplifying, detecting, and/or-cloning nucleic acid sequences. US Patent 4,683,195 dated Jul 28 1987

Mullis KB. Process for amplifying nucleic acid sequences. US Patent 4,683,202 dated Jul 28 1987