Neuroretinas were dissected from normal quail embryos, dissociated and immortalized by infection with Rous sarcoma virus (RSV) mutant ts NY-68 to establish the QNR ts NY-68 mixed cell line.
QNR ts NY-68 was subsequently cloned to establish the QNR/D cell line. The cells are routinely maintained at 38.5 to 39C. The permissive temperature for transformation is 36C.
Neuroretina (NR) is an evagination of the central nervous system (CNS) which is composed of photoreceptors; glial (Muller) cells; horizontal, bipolar, amacrine; and ganglion neuronal cells.
The cell line displays properties of amacrine and/or ganglion cells.
QNR/D cells can generate tetrodotoxin(TTX)-inhibitable action potentials on electrical stimulation. They have high glutamic acid decarboxylase (GAD) activity and they bind monoclonal antibodies raised against chick embryo neuroretina.
QNR/D cells (ATCC CRL-2532)
and QNR/K2 cell (ATCC CRL-2533)
were transplanted into chicken embryo eyes. Implanted QNR/D cells migrate only to the retinal ganglion and amacrine cell layers and project neurites in the plane of retina.
In contrast, QNR/K2 cells migrate through the ganglion and amacrine layers, locate in the inner nuclear layer, and project processes across the retina.