JAA-F11 (ATCC® CRL-2381)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphoblast  /  Disease: Adenocarcinoma

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphoblast
Product Format frozen
Morphology lymphoblast
Biosafety Level 1
Disease Adenocarcinoma
Applications
The hybridoma cell line JAA-F11 was established in 1995.
The hybridoma produces an antibody (IgG3) that binds the saccharide antigen, Gal beta1-3GalNAc (T-antigen).
The antibody binds to breast adenocarcinoma tissue but not to normal tissues.
It may be useful in tumor studies, germinal center studies and T cell maturation studies.
The JAA-F11 cell line was formed by the fusion of P3X63Ag8.653 mouse myeloma cells with splenocytes from a BALB/c mouse inoculated with a synthetic Gal beta1-3GalNAc-BSA conjugate.
Derivation
The hybridoma cell line JAA-F11 was established in 1995.
Genes Expressed
immunoglobulin; monoclonal antibody; against the saccharide antigen, Gal beta1-3GalNAc (T antigen)
Comments
The hybridoma cell line JAA-F11 was established in 1995.
The hybridoma produces an antibody (IgG3) that binds the saccharide antigen, Gal beta1-3GalNAc (T-antigen).
The saccharide antigen, Gal beta1-3GalNAc is of biologic importance in many systems.
It is a tumor-associated carbohydrate antigen, a temporally expressed antigen in germinal center B cells and cortical T cells, a parasite-associated antigen, a spermatozoa vitality marker and an antigen on aged red blood cells.
The antibody binds to breast adenocarcinoma tissue but not to normal tissues.
It may be useful in tumor studies, germinal center studies and T cell maturation studies.
The JAA-F11 cell line was formed by the fusion of P3X63Ag8.653 mouse myeloma cells with splenocytes from a BALB/c mouse inoculated with a synthetic Gal beta1-3GalNAc-BSA conjugate.
Complete Growth Medium Dulbecco's modified Eagle's Medium with 4 mM L-glutamine adjusted to contain 4.5 g/L glucose and 1.5 g/L sodium bicarbonate and supplemented with: 0.1 mM NEAA, 1.0% MEM Vitamins and 10% fetal bovine serum
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 2 X 10 exp5 and 5 X 10 exp5 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG3; kappa light chain
Name of Depositor K Rittenhouse-Diakun
Year of Origin 1995
References

Rittenhouse-Diakun K, et al. Development and characterization of monoclonal antibody to T-antigen: (gal beta1-3GalNAc-alpha-O). Hybridoma 17: 165-173, 1998. PubMed: 9627057

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Rittenhouse-Diakun K, et al. Development and characterization of monoclonal antibody to T-antigen: (gal beta1-3GalNAc-alpha-O). Hybridoma 17: 165-173, 1998. PubMed: 9627057